Glucose effect in tgl mutant of Escherichia col K12 defective in methyl-alpha-D-glucoside transport
| Autor: | Genrich I. Bourd, Raisa S. Erlagaeva, Vladimir N. Gershanovitch, Marina V. Shulgina, Tatyana N. Bolshakova |
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| Rok vydání: | 1977 |
| Předmět: |
Mutant
Catabolite repression Biological Transport Active Biology medicine.disease_cause Biochemistry Enzyme Repression Thiogalactosides Species Specificity Glucoside transport medicine Cyclic AMP Escherichia coli Inducer Amino Acids Psychological repression Methylglycosides Methylglucosides PEP group translocation Galactosidases Kinetics Glucose Mutation |
| Zdroj: | European journal of biochemistry. 72(1) |
| ISSN: | 0014-2956 |
| Popis: | 1. The dependence of the rate of accumulation of methyl-alpha-D-glucoside on its extracellular concentration was studied in the tgl mutant of Escherichia coli K12, isolated earlier. It has been shown that the kinetics of methyl-alpha-D-glucoside transport differ sharply from those in wild-type bacteria. 2. The beta-galactosidase synthesis in tgl strain is much less sensitive both to permanent and transient glucose catabolite repression. The level of cyclic AMP in mutant cells under the conditions of glucose catabolite repression is several times higher than in the parent strain. 3. The tgl mutation does not affect the manifestation of catabolite inhibition and inducer exclusion with glucose. 4. The data obtained are discussed in the light of a hypothesis concerning the existence of two sites, binding and pecific enzyme II of the phosphoenolpyruvate-dependent phosphotransferase system. The tgl mutation alters the first site, and the second one is damaged by the pgt mutation. 5. It is suggested that the products of the tgl and gpt genes are necessary for the manifestation of the phenomena of glucose permanent and transient repression. The effects of catabolite inhibition and inducer exclusion are realized irrespective of the existence or absence of the tgl product. |
| Databáze: | OpenAIRE |
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