The host immune enhancing agent Korean red ginseng oil successfully attenuates Brucella abortus infection in a murine model
| Autor: | Suk Kim, Man Hee Rhee, Soo Jong Park, Hu Jang Lee, Yi-Seong Kwak, Huynh Tan Hop, Wongi Min, Tran Xuan Ngoc Huy, Lauren Togonon Arayan, Kwang Dong Kim, Alisha Wehdnesday Bernardo Reyes |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_treatment 030106 microbiology Brucella abortus Panax Spleen Nitric Oxide Brucellosis Microbiology Interferon-gamma Mice 03 medical and health sciences Ginseng Immune system Adjuvants Immunologic In vivo Drug Discovery Oils Volatile medicine Animals Plant Oils Interferon gamma Cells Cultured Pharmacology Tumor Necrosis Factor-alpha business.industry Fatty Acids Phytosterols In vitro 030104 developmental biology medicine.anatomical_structure Cytokine Immunology Tumor necrosis factor alpha business medicine.drug |
| Zdroj: | Journal of Ethnopharmacology. 198:5-14 |
| ISSN: | 0378-8741 |
| DOI: | 10.1016/j.jep.2016.12.026 |
| Popis: | Ethnopharmacological relevance Panax ginseng Meyer (Araliaceae), is one of the most valuable traditional Chinese medicines and is used for the treatment of various human diseases. In this study, we elucidated the protective mechanism of the essential oil from Korean red ginseng (RGO) against Brucella infection. Materials and methods The effects of RGO on Brucella abortus viability, NO production, uptake and intracellular growth in macrophages were investigated. Mice were intraperitoneally infected with B. abortus and orally treated with RGO for 14 days. The weights and bacterial numbers from each spleen were monitored, and the sera were evaluated for cytokine production. Results B. abortus viability was not affected, whereas NO production, internalization and intracellular replication were inhibited in RGO-treated macrophages. Bacterial adherence, F-actin polymerization and MAPK signaling protein phosphorylation (ERK1/2, JNK and p38α) were reduced and the co-localization of B. abortus- containing phagosomes with LAMP-1 was augmented in RGO-treated cells compared to untreated cells. RGO displayed protective effects against cell damage by inhibiting nitrite production during B. abortus infection in macrophages. Moreover, the spleen weight and bacterial burden were lower in the RGO-treated group than in the control group. The uninfected RGO-treated mice displayed increased TNF-α and IFN-γ production, whereas the B. abortus- infected RGO-treated mice showed reduced IL-10 production compared to the control. Conclusion RGO exhibits protective effects against B. abortus infection in vitro and in vivo, which emphasize the beneficial effects of RGO in the prevention and treatment of brucellosis. |
| Databáze: | OpenAIRE |
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