Continuous volumetric imaging via an optical phase-locked ultrasound lens
| Autor: | Lingjie Kong, Charles P. Lin, Jianyong Tang, Tim Lämmermann, Ronald N. Germain, Justin P. Little, Yang Yu, Meng Cui |
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| Rok vydání: | 2015 |
| Předmět: |
Optics and Photonics
Materials science Neutrophils Green Fluorescent Proteins Phase (waves) Mice Transgenic Image processing Biochemistry Article law.invention Mice law Oscillometry Microscopy Image Processing Computer-Assisted Fluorescence microscope Animals Molecular Biology Cerebral Cortex business.industry Lasers Ultrasound Neurosciences Brain Dendritic Cells Cell Biology Laser Lens (optics) Microscopy Fluorescence Multiphoton Microscopy Fluorescence Immune System Calibration Calcium Lymph Nodes Microglia business Algorithms Preclinical imaging Biotechnology Biomedical engineering |
| Zdroj: | Nature methods |
| ISSN: | 1548-7105 1548-7091 |
| Popis: | In vivo imaging at high spatiotemporal resolution holds the key to the fundamental understanding of complex biological systems. Integrating an optical phase-locked ultrasound lens into a conventional two-photon fluorescence microscope, we achieved microsecond scale axial scanning, which enabled high-speed volumetric imaging. We applied this system to multicolor volumetric imaging of fast processes, including calcium dynamics in the cerebral cortex of behaving mice, and transient morphology changes and trafficking of immune cells. |
| Databáze: | OpenAIRE |
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