Protein kinase CK2 is implicated in early steps of the differentiation of pre-adipocytes into adipocytes
| Autor: | Mathias Montenarh, Katja B. Kostelnik, Nadine Wilhelm, Claudia Götz |
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| Rok vydání: | 2011 |
| Předmět: |
medicine.medical_specialty
animal structures Cellular differentiation Clinical Biochemistry Gene Expression Anthraquinones Glycerolphosphate Dehydrogenase Biology Gene Expression Regulation Enzymologic MAP2K7 Mice Internal medicine 3T3-L1 Cells Gene expression medicine Adipocytes Animals Amino Acid Sequence Phosphorylation Protein kinase A Casein Kinase II Molecular Biology Regulation of gene expression Kinase fungi Cell Differentiation Cell Biology General Medicine Lipid Metabolism Peptide Fragments Cell biology Endocrinology Adipogenesis embryonic structures Benzimidazoles Protein Processing Post-Translational |
| Zdroj: | Molecular and cellular biochemistry. 365(1-2) |
| ISSN: | 1573-4919 |
| Popis: | Protein kinase CK2 is a ubiquitously expressed serine/threonine kinase which is composed of two catalytic α- or α′-subunits and two non-catalytic β-subunits. CK2 has been shown to be implicated in embryogenesis, spermatogenesis, and the development of certain organs but its role in basal differentiation processes is only sparsely analyzed. 3T3-L1 cells, which are murine pre-adipocytes, can be induced to differentiate into mature adipocytes within 2 weeks using a combination of insulin, dexamethasone, and isobutylmethylxanthine. We found that the activity of CK2 slightly increases until day 6 and subsequently, decreases in fully differentiated adipocytes. The decrease in activity goes along with a lower expression of all the three subunits of CK2. After inhibition of CK2 with 2-dimethylamino-4,5,6,7-tetrabromo-1H-benzimidazole (DMAT) or 1,2,5,8-tetrahydroxyanthraquinone (quinalizarin), before day 6, 3T3-L1 cells did not differentiate into adipocytes; inhibition of CK2 after day 6 had no effect on the differentiation process. These results indicated a role of CK2 in early events of the differentiation process and that CK2 is dispensable for late stages of differentiation. |
| Databáze: | OpenAIRE |
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