Effects of triclosan (TCS) on hormonal balance and genes of hypothalamus-pituitary- gonad axis of juvenile male Yellow River carp (Cyprinus carpio)

Autor: Wanguang Chen, Fan Wang, Wei Wang, Ruijie Xu, Fei Liu
Rok vydání: 2018
Předmět:
Male
0301 basic medicine
Health
Toxicology and Mutagenesis
Hepatopancreas
Estrogen receptor
010501 environmental sciences
01 natural sciences
Cyprinus
Gonadotropin-Releasing Hormone
Vitellogenins
Anti-Infective Agents
Testosterone
Aromatase
Estradiol
biology
Reproduction
General Medicine
Anatomy
Pollution
medicine.anatomical_structure
Receptors
Estrogen
Pituitary Gland
Gonadotropin
endocrine system
medicine.medical_specialty
Carps
Environmental Engineering
Gonad
medicine.drug_class
Hypothalamus
Endocrine System
Enzyme-Linked Immunosorbent Assay
Real-Time Polymerase Chain Reaction
03 medical and health sciences
Vitellogenin
Internal medicine
medicine
Animals
Environmental Chemistry
RNA
Messenger
Gonads
Carp
0105 earth and related environmental sciences
fungi
Public Health
Environmental and Occupational Health
General Chemistry
biology.organism_classification
Hormones
Triclosan
030104 developmental biology
Endocrinology
biology.protein
Water Pollutants
Chemical
Zdroj: Chemosphere. 193:695-701
ISSN: 0045-6535
DOI: 10.1016/j.chemosphere.2017.11.088
Popis: Triclosan (TCS) is a broad spectrum antimicrobial agent which has been widely dispersed and determinated in the aquatic environment. However, the effects of TCS on reproductive endocrine in male fish are poorly understood. In this study, male Yellow River carp (Cyprinus carpio) were exposed to 0, 1/5, 1/10 and 1/20 LC50 (96 h LC50 of TCS to carp) TCS under semi-static conditions for 42 d. Vitellogenin (Vtg), 17β-estradiol (E2), testosterone(T), gonadotropin (GtH), and gonadotropin-releasing hormone (GnRH) levels were measured by enzyme-linked immunosorbent assay (ELISA). Meanwhile, we also examined the mRNA expressions of aromatase, GtHs-β, GnRH, estrogen receptor (Er), and androgen receptor (Ar) by quantitative Real-time Polymerase Chain Reaction (qRT-PCR). TCS induced Vtg levels of hepatopancreas, E2 levels of serum, and inhibited Ar and Er mRNA levels, suggesting that the induction of Vtg production by TCS was indirectly caused by non-Er pathways. TCS-induced Vtg levels by interfering with the reproductive axis at plenty of latent loci of male carps: (a) TCS exposure increased the aromatase mRNA expression of hypothalamus and gonad aromatase, consequently increasing serum concentrations of E2 to induce Vtg in hepatopancreas; (b) TCS treatment changed GtH-β and GnRH mRNA expression and secretion, causing the disturbance of reproductive endocrine; (c) TCS exposure decreased Ar mRNA levels, indicating potential Ar-mediated antiandrogen action. These mechanisms showed that TCS may induce Vtg production in male carp by non-Er-mediated pathways.
Databáze: OpenAIRE
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