Regulatory effects of cytokines and cyclosporine A on peripheral blood mononuclear cells from stable multiple sclerosis patients
| Autor: | Alfredo Prieto, Miguel Angel Alvarez-Mon, C. Guillen, A. Gimeno, M. de la Piedra, J. C. Alvarez-Cermeño |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Adult
Male medicine.medical_specialty Multiple Sclerosis medicine.drug_class medicine.medical_treatment Immunology Alpha interferon Toxicology Monoclonal antibody Lymphocyte Activation Peripheral blood mononuclear cell Internal medicine medicine Immunology and Allergy Humans Phytohemagglutinins Interferon alfa Pharmacology business.industry Multiple sclerosis Interleukin Receptors Interleukin-2 General Medicine Middle Aged medicine.disease In vitro Cytokine Endocrinology Cyclosporine Leukocytes Mononuclear Cytokines Interleukin-2 Tetradecanoylphorbol Acetate Female business Immunosuppressive Agents medicine.drug |
| Zdroj: | Immunopharmacology and immunotoxicology. 21(3) |
| ISSN: | 0892-3973 |
| Popis: | The proliferative response (PR) of peripheral blood mononuclear cells (PBMC) to lectins such as phytohemaglutinin (PHA), anti-CD3 monoclonal antibodies such as OKT-3 or phorbol esters such as tetradecanoyl-phorbol 13-acetate (TPA) was investigated in 18 stable multiple sclerosis (MS) patients (9 untreated and 9 treated patients) and 10 healthy controls. PBMC from untreated MS patients showed a significantly higher PR to PHA than healthy controls. The PR of PHA, anti-CD3 or TPA stimulated PBMC from treated patients was lower than that from untreated MS patients. Mitogen stimulated PBMC from untreated patients shown both increased sensitivity to the stimulatory effect of IL-2 and increased resistance to the inhibitory effect of IL-10 and IFN-alpha. The addition of IL-2 increased the PR in PHA-stimulated PBMC from untreated MS patients, but not in those from treated MS patients and healthy controls. Mitogen stimulated cells from untreated patients were more resistant to the inhibitory effect of IL-10 and IFN-alpha than PBMC from either treated MS patients or healthy controls. Cyclosporine A (CsA) inhibited the PR and the expression of activation antigens induced by PHA in PBMC from the three groups of subjects. This inhibitory effect of CsA have was enhanced by the addition of IFN-alpha. |
| Databáze: | OpenAIRE |
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