Phase separation of Epstein-Barr virus EBNA2 protein reorganizes chromatin topology for epigenetic regulation
| Autor: | Ranran Dai, Yongchang Zhu, Bo Lei, Litao Qin, Delong Feng, Xidong Ye, Zhaoqiang Li, Yan Zhang, Bingtao Hao, Xin Wang, Shixiu Liao, Yiting Yang, Wei Xue |
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| Rok vydání: | 2021 |
| Předmět: |
Herpesvirus 4
Human QH301-705.5 viruses Medicine (miscellaneous) Biology Virus-host interactions medicine.disease_cause Topology Article General Biochemistry Genetics and Molecular Biology Virus Epigenesis Genetic Viral Proteins Transactivation immune system diseases hemic and lymphatic diseases Gene expression medicine Transcriptional regulation Humans Herpes virus Epigenetics Biology (General) virus diseases biochemical phenomena metabolism and nutrition Epstein–Barr virus Chromatin HEK293 Cells Acetylation Protein aggregation General Agricultural and Biological Sciences |
| Zdroj: | Communications Biology Communications Biology, Vol 4, Iss 1, Pp 1-14 (2021) |
| ISSN: | 2399-3642 |
| DOI: | 10.1038/s42003-021-02501-7 |
| Popis: | Epstein-Barr virus nuclear antigen 2 (EBNA2) is a transactivator of viral and cellular gene expression, which plays a critical role in the Epstein-Barr virus-associated diseases. It was reported that EBNA2 regulates gene expression by reorganizing chromatin and manipulating epigenetics. Recent studies showed that liquid-liquid phase separation plays an essential role in epigenetic and transcriptional regulation. Here we show that EBNA2 reorganized chromatin topology to form accessible chromatin domains (ACDs) of the host genome by phase separation. The N-terminal region of EBNA2, which is necessary for phase separation, is sufficient to induce ACDs. The C-terminal domain of EBNA2 promotes the acetylation of accessible chromatin regions by recruiting histone acetylase p300 to ACDs. According to these observations, we proposed a model of EBNA2 reorganizing chromatin topology for its acetylation through phase separation to explain the mechanism of EBNA2 hijacking the host genome by controlling its epigenetics. Yang et al find that phase separation of the Epstein-Barr virus nuclear antigen 2 (EBNA2) is involved in the formation of accessible chromatin domains of the host genome. They also find that EBNA2 recruits histone acetyltransferase to promote histone acetylation on accessible chromatin regions and regulate gene expression and that these two functions are performed by the N- and Cterminus respectively. |
| Databáze: | OpenAIRE |
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