Regional variation of the alphabeta T cell repertoire in the colon of healthy individuals and patients with Crohn's disease
| Autor: | Andreas Hennemann, Carsten Lambert, Wolfgang Holtmeier, Ekkehard May, Martin Zeitz, Rainer Duchmann |
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| Rok vydání: | 2002 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Adolescent Colon T cell Receptors Antigen T-Cell alpha-beta Immunology Molecular Sequence Data Complementarity determining region Biology Inflammatory bowel disease Pathogenesis Intestinal mucosa Crohn Disease medicine Immunology and Allergy Humans Amino Acid Sequence Intestinal Mucosa Crohn's disease Repertoire T-cell receptor General Medicine Middle Aged medicine.disease Inflammatory Bowel Diseases Complementarity Determining Regions medicine.anatomical_structure Genes T-Cell Receptor beta Sequence Analysis |
| Zdroj: | Human immunology. 63(6) |
| ISSN: | 0198-8859 |
| Popis: | Clonally expanded T cells might be involved in the pathogenesis of Crohn's disease (CD). To test the impact of CD on the regional distribution of expanded T cells, this study analyzed the T cell receptor beta (TCRB) repertoire within colonic biopsy specimens from 12 CD patients and 6 noninflammatory controls by TCR spectratyping. Migration characteristics of dominant CDR3 bands from different sites of the normal mucosa suggested focal, segmental, or ubiquitous spreading of individual expanded clones. Similar patterns were observed when inflamed and noninflamed areas of the colon of CD patients were compared, suggesting that regional expansion of T cells was more closely related to anatomic proximity than to local inflammatory activity. CDR3-sequence analysis of TCRBV12+ T cells, which were selectively expanded in the inflamed colon of 3 CD patients, failed to reveal a public CDR3 motif. Our data indicate the existence of distinct patterns of regional T cell expansions in the normal gut mucosa, which are not significantly disrupted by chronic intestinal inflammation. This does not exclude a pathogenic role of expanded T cells in CD through more subtle changes, but emphasizes the need to distinguish them from a discontinuous distribution of clonally expanded T cells in normal colon. |
| Databáze: | OpenAIRE |
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