Polymorphism and evolution of collagenolytic serine protease genes in crustaceans
| Autor: | Alain Van Wormhoudt, Daniel Sellos |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Molecular Sequence Data
Biophysics Polymerase Chain Reaction Biochemistry Arthropod Proteins Evolution Molecular Exon Structural Biology Crustacea Decapoda Complementary DNA Animals Chymotrypsin Amino Acid Sequence Collagenases Molecular Biology Gene Peptide sequence Serine protease Genetics Polymorphism Genetic Base Sequence biology Intron Nucleic acid sequence Isoenzymes genomic DNA Genes biology.protein Serine Proteases Sequence Alignment |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 1432:419-424 |
| ISSN: | 0167-4838 |
| DOI: | 10.1016/s0167-4838(99)00121-1 |
| Popis: | Two genomic DNA fragments encoding crustacean collagenolytic serine protease genes show coding fragments that span 1522–1526 base pairs and contain seven exons encoding the complete amino acid sequence of two enzymes, CHYA and CHYB. As in serine protease genes from other organisms, the region coding for the residues around the active site is split by two introns. Although the introns differ from those of other organisms in size and nucleotide sequence, their number and location are more or less the same as found in mammalian chymotrypsin or elastase genes that evolved lately, but different for trypsin genes. Meanwhile, the junction that occurs between the propeptide and the maturation site is only found in the shrimp genes. This is also the case for the junction located 13 amino acids after the active site aspartic acid in these genes. Between 40 and 50 copies of the genes are reported by Southern analysis. Seven different genes within ChyA Pv family present 0–6% base changes, whereas five different genes belonging to ChyB Pv family show changes of up to 27% in the short studied portion of exon 4. This last family presents a mosaic organization of the coding parts, which are also expressed in the hepatopancreas of the shrimp as the variant PVC5 cDNA. |
| Databáze: | OpenAIRE |
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