ÓLEOS ESSENCIAIS DE Blepharocalyx, Nectandra e Piper: ATIVIDADE SOBRE FUNGOS APODRECEDORES DE MADEIRA

Autor: Nadia Helena Bianchini, Fernando Nunes Gouveia, Marcelo Fontana Da Silveira, Carlos Garrido Pinheiro, Berta Maria Heinzmann
Jazyk: portugalština
Rok vydání: 2022
Předmět:
Zdroj: Nativa; v. 10 n. 3 (2022); 417-422
Nativa; Vol. 10 No. 3 (2022); 417-422
Nativa (Sinop)
Universidade Federal de Mato Grosso (UFMT)
instacron:UFMT
ISSN: 2318-7670
Popis: This study aimed to use digital image processing to evaluate the antifungal activity of essential oils (EOs) from Blepharocalyx salicifolius (Myrtaceae), Nectandra grandiflora (Lauraceae), Nectandra megapotamica (Lauraceae) and Piper gaudichaudianum (Piperaceae) on wood decay fungi. A white-rot fungus, Trametes versicolor, and a brown-rot fungus, Gloeophyllum trabeum, were selected. The EOs were extracted by hydrodistillation and chemically characterized by gas chromatography coupled to mass spectrometry (GC-MS). The antifungal activity of the EOs was evaluated by the Petri plate diffusion test. The inhibition verification was calculated with the aid of digital image processing, using ImageJ software. The predominant compounds of the B. salicifolius and P. gaudichaudianum extractives were eucalyptol and dillapiol, respectively. For the species of Nectandra, bicyclogermacrene was the most representative constituent of the N. megapotamica EO, whereas dehydrofuquinone was the major constituent of the EOs from N. grandiflora leaves and fruits. The highest growth inhibition of T. versicolor was caused by N. megapotamica EO. The highest inhibition percentages of G. trabeum were provided by P. gaudichaudianum and B. salicifolius extractives. Digital image processing was an effective tool for the accurate analysis of fungal growth. A busca por produtos naturais que possam substituir preservantes da madeira sintéticos é crescente. Dentre estes produtos, estão os óleos essenciais (OEs). Portanto, o objetivo deste estudo foi avaliar a atividade antifúngica dos OEs de Blepharocalyx salicifolius, Nectandra grandiflora, Nectandra megapotamica e Piper gaudichaudianum, sobre os fungos apodrecedores de madeira Trametes versicolor e Gloeophyllum trabeum. Os OEs foram extraídos por hidrodestilação e foram caracterizados quimicamente. A atividade antifúngica dos OEs foi avaliada pelo teste de difusão em placas de Petri. A verificação da inibição foi calculada com auxílio do software ImageJ®. Os componentes majoritários dos OEs de B. salicifolius e P. gaudichaudianum foram eucaliptol e dilapiol, respectivamente. Biciclogermacreno foi o constituinte majoritário do óleo essencial (OE) de folhas de N. megapotamica, enquanto que a deidrofuquinona foi majoritária nos OEs de folhas e frutos de N. grandiflora. A maior inibição do crescimento de T. versicolor foi causada pelo OE de N. megapotamica, com 31,4%. Os maiores percentuais de inibição observados sobre o crescimento de G. trabeum foram proporcionados pelos extrativos de P. gaudichaudianum e B. salicifolius, com 13,9 e 7,5%, respectivamente. Contudo, o resultado deste último não diferiu dos demais OEs avaliados. Os resultados indicaram que o extrativo de espécie de Nectandra que apresentou maior efeito sobre T. versicolor foi N. megapotamica. Entretanto, este gênero não foi tão eficiente contra G. trabeum. Palavras-chave: biciclogermacreno; deidrofuquinona; dilapiol; eucaliptol; Gloeophyllum trabeum; Trametes versicolor. Blepharocalyx, Nectandra and Piper essential oils: activity on wood-decay fungi ABSTRACT: The search for natural products that can replace synthetic wood preservatives is growing. Among these products are essential oils (EOs). Therefore, the aim of this study was to evaluate the antifungal activity of EOs from Blepharocalyx salicifolius , Nectandra grandiflora , Nectandra megapotamica and Piper gaudichaudianum on the wood-rotting fungi Trametes versicolor and Gloeophyllum trabeum. The EOs were extracted by hydrodistillation and chemically characterized. The antifungal activity of the EOs was evaluated by the Petri dish diffusion test. The inhibition verification was calculated using ImageJ® software. The major components of the EOs of B. salicifolius and P. gaudichaudianum were eucalyptol and dillapiol, respectively. Bicyclogermacrene was the major constituent of N. megapotamica leaves essential oil (EO), while dehydrofuquinone was the major constituent of the EOs from N. grandiflora leaves and fruits. The highest growth inhibition of T. versicolor was caused by N. megapotamica EO, with 31.4%. The highest inhibition percentages of G. trabeum growth were provided by P. gaudichaudianum and B. salicifolius extractives, with 13.9 and 7.5%, respectively. However, the result of the latter did not differ from the other EOs evaluated. The results indicated that, from the extractives of Nectandra species, the greatest effect on T. versicolor was detected to N. megapotamica EO. However, this genus was not so efficient against G. trabeum. Keywords: bicyclogermacrene; dehydrofuquinone; dillapiole; eucalyptol; Gloeophyllum trabeum; Trametes versicolor.
Databáze: OpenAIRE
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