Glutamine metabolism regulates autophagy-dependent mTORC1 reactivation during amino acid starvation
| Autor: | Arthur Yi Loong Sim, Hayden Weng Siong Tan, Yun Chau Long |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Transamination Science Glutamine Immunoblotting General Physics and Astronomy mTORC1 Mechanistic Target of Rapamycin Complex 1 Biology Article General Biochemistry Genetics and Molecular Biology Autophagy-Related Protein 5 Cell Line Myoblasts 03 medical and health sciences Autophagy Animals Humans Amino Acids lcsh:Science Cells Cultured Essential amino acid Mice Knockout chemistry.chemical_classification Multidisciplinary Glutaminolysis Kinase Hep G2 Cells General Chemistry Fibroblasts Embryo Mammalian Amino acid 030104 developmental biology chemistry Biochemistry lcsh:Q biological phenomena cell phenomena and immunity Lysosomes |
| Zdroj: | Nature Communications, Vol 8, Iss 1, Pp 1-10 (2017) Nature Communications |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-017-00369-y |
| Popis: | Activation of autophagy and elevation of glutamine synthesis represent key adaptations to maintain amino acid balance during starvation. In this study, we investigate the role of autophagy and glutamine on the regulation of mTORC1, a critical kinase that regulates cell growth and proliferation. We report that supplementation of glutamine alone is sufficient to restore mTORC1 activity during prolonged amino acid starvation. Inhibition of autophagy abolishes the restorative effect of glutamine, suggesting that reactivation of mTORC1 is autophagy-dependent. Inhibition of glutaminolysis or transamination impairs glutamine-mediated mTORC1 reactivation, suggesting glutamine reactivates mTORC1 specifically through its conversion to glutamate and restoration of non-essential amino acid pool. Despite a persistent drop in essential amino acid pool during amino acid starvation, crosstalk between glutamine and autophagy is sufficient to restore insulin sensitivity of mTORC1. Thus, glutamine metabolism and autophagy constitute a specific metabolic program which restores mTORC1 activity during amino acid starvation. mTORC1 is a critical kinase that regulates cell growth and proliferation. Here the authors show that glutamine metabolism is sufficient to restore mTORC1 activity during prolonged amino acid starvation in an autophagy-dependent manner. |
| Databáze: | OpenAIRE |
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