Isolation and Characterisation of a Dinucleotide Microsatellite Set for a Parentage and Biodiversity Study in Domestic Guinea Pig(Cavia Porcellus)
| Autor: | Juan Vicente Delgado, Jose Luis Vega-Pla, Vincenzo Landi, Amparo Martínez Martínez, Diana Aviles |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Genetic diversity biology 0402 animal and dairy science Biodiversity Cavia Zoology 04 agricultural and veterinary sciences biology.organism_classification 040201 dairy & animal science Guinea pig Animal protein Power of exclusion 03 medical and health sciences STRs 030104 developmental biology Cavia porcellus Genetic resources South american Microsatellite Animal Science and Zoology lcsh:Animal culture lcsh:SF1-1100 |
| Zdroj: | Italian Journal of Animal Science, Vol 14, Iss 4 (2015) |
| ISSN: | 1828-051X |
| DOI: | 10.4081/ijas.2015.3960 |
| Popis: | The domestic guinea pig is a valuable genetic resource because it is part of local folklore and food tradition in many South American countries. The economic importance of the guinea pig is due to its high feed efficiency and the quality of animal protein produced. For these reasons, our study is aimed to design a complete dinucleotide microsatellite marker set following international recommendation to assess the genetic diversity and genealogy management of guinea pigs. We selected a total of 20 microsatellites, looking for laboratory efficiency and good statistical parameters. The set was tested in 100 unrelated individuals of guinea pigs from Ecuador, Peru, Colombia, Bolivia and Spain. Our results show a high degree of polymorphisms with a total of 216 alleles and a mean number of 10.80±3.49 for markers with a combined exclusion probability of 0.99. |
| Databáze: | OpenAIRE |
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