Reproductive parameters of double transgenic zebrafish (Danio rerio) males overexpressing both the growth hormone (GH) and its receptor (GHR)
| Autor: | Bruna Félix Nornberg, Diego Martins Pires, Carine Dahl Corcini, Antonio Sergio Varela Junior, Jessica Ribeiro Pereira, Daniela Volcan Almeida, Luis Fernando Marins, Ana Cecilia Gomes Silva |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Transgene Danio Growth hormone receptor Biology Animals Genetically Modified 03 medical and health sciences Internal medicine Genetics medicine Animals Receptor Muscle Skeletal Zebrafish Reproduction 0402 animal and dairy science Gene Transfer Techniques 04 agricultural and veterinary sciences Receptors Somatotropin biology.organism_classification 040201 dairy & animal science Sperm Transgenesis 030104 developmental biology Endocrinology Growth Hormone Animal Science and Zoology Agronomy and Crop Science Biotechnology Hormone |
| Zdroj: | Transgenic research. 26(1) |
| ISSN: | 1573-9368 |
| Popis: | Growth hormone (GH) transgenesis presents a high potential application in aquaculture. However, excess GH may have serious consequences due to pleiotropic actions. In order to study these effects in zebrafish (Danio rerio), two transgenic lines were developed. The first expresses GH ubiquitously and constitutively (F0104 line), while the second expresses the GH receptor in a muscle-specific manner (Myo-GHR line). Results from the F0104 line showed accelerated growth but increased reproductive difficulties, while Myo-GHR did not show the expected increase in muscle mass. Since the two lines appeared to display complementary characteristics, a double transgenic (GH/GHR) was created via crossing between them. This double transgenic displayed accelerated growth, however reproductive parameters remained uncertain. The objective of the present study was to determine the reproductive capacity of males of this new line, by evaluating sperm parameters, expression of spermatogenesis-related genes, and reproductive tests. Double transgenics showed a strong recovery in almost all sperm parameters analyzed when compared to the F0104 line. Gene expression analyses revealed that Anti-Mullerian Hormone gene (amh) appeared to be primarily responsible for this recovery. Reproductive tests showed that double transgenic males did not differ from non-transgenics. It is possible that GHR excess in the muscle tissues of double transgenics may have contributed to lower circulating GH levels and thus reduced the negative effects of this hormone with respect to reproduction. Therefore, it is clear that GH-transgenesis technology should take into account the need to obtain adequate levels of circulating hormone in order to achieve maximum growth with minimal negative side effects. |
| Databáze: | OpenAIRE |
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