Sodium-hydrogen exchange inhibitors improve postischaemic recovery of function in the perfused rabbit heart
| Autor: | Morris Karmazyn, Mary Lee Myers, Sanjiv Mathur, Guang-Hue Li |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Male
Sodium-Hydrogen Exchangers Physiology Metabolite Myocardial Ischemia Ischemia Myocardial Reperfusion Pharmacology Amiloride chemistry.chemical_compound Reperfusion therapy Physiology (medical) medicine Animals Lagomorpha biology business.industry Heart medicine.disease biology.organism_classification Perfusion chemistry Anesthesia biology.protein Coronary perfusion pressure Creatine kinase Rabbits Cardiology and Cardiovascular Medicine business medicine.drug |
| Zdroj: | Cardiovascular Research. 29:209-214 |
| ISSN: | 0008-6363 |
| DOI: | 10.1016/s0008-6363(96)88572-5 |
| Popis: | Objective: The aim was to examine the effects of the Na+/H+ exchange inhibitors amiloride and methylisobutyl amiloride (MIA) in buffer perfused rabbit hearts subjected to one hour of normothermic ischaemia (37 °C) followed by reperfusion. Methods: Experiments were carried out in five groups of Langendorff perfused rabbit hearts: (1) control, (2) amiloride, and (3) MIA (agents in both the preischaemic and reperfusion perfusate), (4) amiloride-R and (5) MIA-R (agents added at reperfusion only). Functional evaluation included serial measurement of resting tension, force, rates of ventricular force development and relaxation, and coronary perfusion pressure. Samples of coronary effluent were obtained for creatine kinase assay and hearts were freeze clamped for metabolite assays. Results: Reperfusion resulted in a marked increase in resting tension in group (1) which was statistically significant compared to groups (2) and (3). Groups (2) and (3) also showed significantly improved recovery of ventricular force, rate of force development, and rate of ventricular relaxation. Addition of either agent only during reperfusion failed to produce a significant beneficial effect. There were no significant differences among the groups with respect to postreperfusion creatine kinase release or end reperfusion metabolite levels. Conclusion: This study shows for the first time that both of the Na+/H+ exchange inhibitors amiloride and MIA produce improved recovery of ventricular function in rabbit hearts subjected to ischaemia and reperfusion, although the beneficial effect was not obtained with drug administration at the time of reperfusion only. |
| Databáze: | OpenAIRE |
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