Evaluation of Horn Flies (Diptera: Muscidae) from a Pyrethroid Susceptible Colony for General and Permethrin Esterase Activities
| Autor: | D. D. Oehler, Felix D. Guerrero, Diane M. Kammlah, John H. Pruett |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Male
Insecticides Pesticide resistance Naphthols Esterase Microbiology Toxicology chemistry.chemical_compound Sex Factors Pyrethrins parasitic diseases medicine Animals Permethrin Pyrethroid Ecology biology Hydrolysis Muscidae fungi Age Factors Esterases General Medicine biology.organism_classification Blood meal Enzyme assay Haematobia irritans chemistry Insect Science biology.protein Female medicine.drug |
| Zdroj: | Journal of Economic Entomology. 93:920-924 |
| ISSN: | 0022-0493 |
| DOI: | 10.1603/0022-0493-93.3.920 |
| Popis: | In this study we describe a nonradioactive single-fly microassay for permethrin hydrolysis. We used this assay with a microplate assay for general esterase activity to evaluate the permethrin hydrolyzing and general esterase activities of aging pyrethroid-susceptible male and female horn flies, Haematobia irritans (L.). We found substantial gender- and age-related differences regarding general esterase activity, permethrin sensitivity, and permethrin hydrolyzing activity within the colony. Extracts of female flies collected 48 h after receiving their first blood meal yielded significantly greater esterase activity than male extracts. Aging female flies were more tolerant of permethrin than were male flies. In addition, a positive correlation was found to exist between the general esterase activity of aging females and their ability to hydrolyze permethrin. |
| Databáze: | OpenAIRE |
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