Isolation and characterisation of monoclonal antibodies against hydrophobic membrane subunit 9 of the yeast mitochondrial H+-ATPase
| Autor: | S. Marzuki, M. J. B. Jean-Francois, Paul J. Hertzog |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Enzyme complex
Antigens Fungal medicine.drug_class ATPase Protein subunit Proteolipids Saccharomyces cerevisiae Biophysics Cross Reactions Monoclonal antibody Biochemistry Epitope Fungal Proteins Epitopes Mice medicine Animals Antibodies Fungal Hybridomas biology ATP synthase Antibodies Monoclonal Cell Biology biology.organism_classification Molecular biology Yeast Mitochondria Proton-Translocating ATPases biology.protein |
| Zdroj: | Biochimica et biophysica acta. 933(1) |
| ISSN: | 0006-3002 |
| Popis: | Five stable lines of myeloma-spleen cell hybrids, producing antibodies against the proteolipid subunit 9 of the yeast mitochondrial H+-ATPase F0-sector, have been isolated by immunizing mice with a proteolipid preparation in the presence of sodium dodecyl sulphate. One of these monoclonal antibodies also reacted with subunit 8 of the enzyme complex indicating a shared epitope. The antibodies did not react with the holo-H+-ATPase, suggesting that their epitopes are shielded by other subunits of the enzyme complex. |
| Databáze: | OpenAIRE |
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