Siderophore production by Staphylococcus aureus and identification of iron-regulated proteins
| Autor: | D. Trivier, Guy R. Martin, M. R. W. Brown, M.-C. Bissinger, R J Courcol |
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| Rok vydání: | 1997 |
| Předmět: |
Staphylococcus aureus
Siderophore Iron Blotting Western Immunology Colony Count Microbial Siderophores medicine.disease_cause Microbiology Mass Spectrometry Bacterial Proteins medicine Humans Polyacrylamide gel electrophoresis biology Molecular mass biology.organism_classification Culture Media Blot Kinetics Chemically defined medium Infectious Diseases Biochemistry Polyclonal antibodies biology.protein Electrophoresis Polyacrylamide Gel Parasitology Bacteria Research Article |
| Zdroj: | Infection and Immunity. 65:1944-1948 |
| ISSN: | 1098-5522 0019-9567 |
| Popis: | Siderophore activity of Staphylococcus aureus was detected in an iron-restricted chemically defined medium. The molecular mass of this siderophore, called aureochelin, was 577 Da. Surface-associated proteins of 120, 88, 57, 35, and 33 kDa were mainly expressed under iron restriction conditions. Results showed a relationship between siderophore production and the existence of the 120- and 88-kDa proteins. Western blotting of surface-associated proteins revealed that these proteins were recognized both by patients sera and polyclonal rabbit serum. |
| Databáze: | OpenAIRE |
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