Cytotoxic abietane diterpenes from Hyptis martiusii Benth
| Autor: | Letícia V. Costa-Lotufo, Edigênia Cavalcante da Cruz Araújo, Raquel Carvalho Montenegro, Mary Anne S. Lima, Cláudia Pessoa, Manoel Odorico de Moraes, Marcelle A. S. Nogueira, Edilberto R. Silveira |
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| Rok vydání: | 2006 |
| Předmět: |
DNA Replication
DNA synthesis Stereochemistry Cell Survival Acridine orange HL-60 Cells Biology Molecular biology Plant Roots General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound chemistry Cell culture Apoptosis Cell Line Tumor Abietanes Cytotoxic T cell Humans Hyptis Ethidium bromide Incubation Abietane |
| Zdroj: | Zeitschrift fur Naturforschung. C, Journal of biosciences. 61(3-4) |
| ISSN: | 0939-5075 |
| Popis: | From roots of Hyptis martiusii Benth. two tanshinone diterpenes were isolated, the new 7β-hydroxy-11,14-dioxoabieta-8,12-diene (1) in addition to the known 7α-acetoxy-12-hydroxy- 11,14-dioxoabieta-8,12-diene (7α-acetoxyroyleanone) (2). Structures of 1 and 2 were established by spectroscopic means. The cytotoxic activity against five cancer cell lines was evaluated. Compounds 1 and 2 displayed considerable cytotoxic activity against several cancer cell lines with IC50 values in the range of 3.1 to 11.5 μg/ml and 0.9 to 7.6 μg/ml, respectively. The cytotoxic activity seemed to be related to inhibition of DNA synthesis, as revealed by the reduction of 5-bromo-2′-deoxyuridine incorporation and induction of apoptosis, as indicated by the acridine orange/ethidium bromide assay and morphological changes after 24 h of incubation in leukemic cells. |
| Databáze: | OpenAIRE |
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