Favorable interleukin-8 induction in human gingival epithelial cells by the antimicrobial peptide LL-37
| Autor: | Thanapat Sastraruji, Watchara Kasinrerk, Sakornrat Khongkhunthian, Nuttapol Chruewkamlow, Suttichai Krisanaprakornkit, Pattanin Montreekachon, Sirikul Nongparn |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Chemokine medicine.medical_treatment Immunology Gingiva Cathelicidin Cathelicidins medicine Immunology and Allergy Humans MTT assay Secretion Interleukin 8 Cytotoxicity Cells Cultured biology Reverse Transcriptase Polymerase Chain Reaction Interleukin-8 Chemotaxis Epithelial Cells General Medicine Molecular biology Cytokine Gene Expression Regulation biology.protein Female Antimicrobial Cationic Peptides |
| Zdroj: | Asian Pacific journal of allergy and immunology. 32(3) |
| ISSN: | 0125-877X |
| Popis: | Background: LL-37, the only member of the antimicrobial peptide cathelicidin family in humans, exerts a variety of biological activities,especially immunomodulation through either direct chemotactic activity or up-regulation of several cytokines and chemokines in various cell types. In this study, we aimed to determine the immunoregulatory effect of LL-37 on Th1/Th2 cytokine expression and production in human gingival epithelial cells (HGECs). Methods: Cultured HGECs were treated with different concentrations of LL-37 for different numbers of times. The cytotoxicity of LL-37 was determined by an MTT assay. Total RNA was isolated for RT-PCR and real-time PCR analyses of cytokine expression. Cell-free culture supernatants were assayed for Th1/Th2 cytokine levels by a cytokine bead array. Results: Out of eleven Th1/Th2 cytokines tested, treatment of HGECs with non-toxic doses of LL- 37 (2-6 μM) significantly raised only IL-8 levels in the cell-free culture supernatants, when compared to control untreated cells (P |
| Databáze: | OpenAIRE |
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