Epitope mapping of new monoclonal antibodies recognizing distinct human FcRII (CD32) isoforms
| Autor: | Kirsten Wissel, Jürgen Frey, Nils Bewarder, Volker Weinrich, Peter Sondermann |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1996 |
| Předmět: |
Gene isoform
Antigenicity medicine.drug_class Molecular Sequence Data Immunology Monoclonal antibody Epitope law.invention Mice Antibody Specificity law Escherichia coli Genetics medicine Animals Humans Amino Acid Sequence Peptide sequence Hybridomas Sequence Homology Amino Acid biology Receptors IgG Antibodies Monoclonal Molecular biology Peptide Fragments Recombinant Proteins Epitope mapping Recombinant DNA biology.protein Antibody Epitope Mapping |
| Popis: | The class II Fc gamma receptors are widely distributed on cells of the immune system. Nevertheless, the exact cell type distribution of the FcRII isoforms is still unclear because of the lack of appropriate antibodies that discriminate between the various isoforms. In this study we describe the generation and characterization of three monoclonal antibodies (MAbs) raised against recombinant human FcRIIb2 as well as a synthetic peptide (amino acids 30-39) of this receptor. Analyses of the isoform specificity of these antibodies using ELISA and Western blots revealed that the MAbs II1A5 (mIgG1) and ID2.7 (mIgM) are pan FcRII antibodies recognizing all known FcRII isoforms. In contrast, the MAb II8D2 (mIgG1) specifically reacts with FcRIIb but not with FcRIIa. The observed antibody reactivities could be confirmed by examination of the exact epitopes using overlapping 15-mer peptides spanning the entire FcRIIb2. So far these antibodies are the only ones described that detect FcRII in Western blots. Moreover, they can be used to analyze the cellular FcRII isoform distribution at the protein level, which was otherwise not possible. |
| Databáze: | OpenAIRE |
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