SGK1-dependent cardiac CTGF formation and fibrosis following DOCA treatment
| Autor: | Peer Wulff, Ulrich Flögel, Karin Klingel, Dan Yang Huang, Florian Lang, Susanne Berchtold, Jürgen Schrader, Agnes Görlach, Nancy D. Dalton, Amanda W. Wyatt, Hartmut Osswald, Jürgen Daut, Florian Grahammer, John Ross, Björn Friedrich, Rachida S. BelAiba, Azeemudeen Hussain, Volker Vallon, Dietmar Kuhl, Reinhard Kandolf |
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| Rok vydání: | 2006 |
| Předmět: |
Male
medicine.medical_specialty Angiotensins Heart Diseases Cardiac fibrosis medicine.drug_class Blood Pressure Protein Serine-Threonine Kinases Spironolactone Biology Immediate-Early Proteins Mice chemistry.chemical_compound Mineralocorticoid receptor Fibrosis Internal medicine Drug Discovery polycyclic compounds medicine Animals Desoxycorticosterone Genetics (clinical) Mineralocorticoid Receptor Antagonists Aldosterone urogenital system Reabsorption Myocardium Connective Tissue Growth Factor NF-kappa B Heart medicine.disease Mice Mutant Strains CTGF Receptors Mineralocorticoid Endocrinology chemistry Mineralocorticoid SGK1 Intercellular Signaling Peptides and Proteins Molecular Medicine Salts hormones hormone substitutes and hormone antagonists |
| Zdroj: | Journal of Molecular Medicine. 84:396-404 |
| ISSN: | 1432-1440 0946-2716 |
| DOI: | 10.1007/s00109-005-0027-z |
| Popis: | The mineralocorticoids aldosterone and deoxycorticosterone acetate (DOCA) stimulate renal tubular salt reabsorption, increase salt appetite, induce extracellular volume expansion, and elevate blood pressure. Cardiac effects of mineralocorticoids include stimulation of matrix protein deposition leading to cardiac fibrosis, which is at least partially due to the direct action of the hormones on cardiac cells. The signaling mechanisms mediating mineralocorticoid-induced cardiac fibrosis have so far remained elusive. Mineralocorticoids have been shown to upregulate the serum- and glucocorticoid-inducible kinase 1 (SGK1), which participates in the effects of mineralocorticoids on renal tubular Na+ reabsorption and salt appetite. To explore the involvement of SGK1 in the pathogenesis of mineralocorticoid-induced cardiac fibrosis, SGK1 knockout mice (sgk1-/-) and wild-type littermates (sgk1+/+) were implanted a 21-day-release 50-mg DOCA pellet and supplied with 1% NaCl in drinking water for 18 days. This DOCA/high-salt treatment increased blood pressure in both genotypes but led to significant cardiac fibrosis only in sgk1+/+ but not in sgk1-/- mice. According to real-time polymerase chain reaction and Western blotting, DOCA/high-salt treatment enhanced transcript levels and protein expression of cardiac connective tissue growth factor (CTGF) only in sgk1+/+ but not in sgk1-/- mice. Furthermore, DOCA (10 microM) upregulated CTGF expression and enhanced CTGF promoter activity in lung fibroblasts isolated from sgk1+/+ but not from sgk1-/- mice, an effect involving spironolactone-sensitive mineralocorticoid receptors and activation of nuclear factor-kappaB (NFkappaB). Our results suggest that SGK1 plays a decisive role in mineralocorticoid-induced CTGF expression and cardiac fibrosis. |
| Databáze: | OpenAIRE |
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