Interaction between activated chemokine receptor 1 and FcεRI at membrane rafts promotes communication and F-actin-rich cytoneme extensions between mast cells
| Autor: | Shoichiro Ono, Santa Jeremy Ono, Freddy Beer, Nimita Fifadara |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
CCR1
Time Factors Immunology Receptors CCR1 Cell Communication G(M1) Ganglioside Transfection Immunoglobulin E Filamentous actin Mice Membrane Microdomains Lymphocyte costimulation medicine Animals Immunology and Allergy Calcium Signaling Mast Cells Cell Shape Lipid raft Cells Cultured Chemokine CCL3 Mice Inbred BALB C biology Receptors IgE Degranulation General Medicine Bridged Bicyclo Compounds Heterocyclic Mast cell Actins Endocytosis Recombinant Proteins Rats Cell biology Protein Transport Cholesterol medicine.anatomical_structure biology.protein Thiazolidines Female Original Research Papers Cytoneme |
| Zdroj: | International Immunology. 22:113-128 |
| ISSN: | 1460-2377 0953-8178 |
| Popis: | Chemokines play important regulatory roles in immunity, but their contributions to mast cell function remain poorly understood. We examined the effects of FcepsilonRI-chemokine receptor (CCR) 1 co-stimulation on receptor localization and cellular morphology of bone marrow-derived mast cells. Whereas FcepsilonRI and CCR1 co-localized at the plasma membrane in unsensitized cells, sensitization with IgE promoted internalization of CCR1 molecules. Co-stimulation of FcepsilonRI and CCR1 with antigen and macrophage inflammatory protein-1alpha was more effective than FcepsilonRI stimulation alone in causing leading edge formation, flattened morphology, membrane ruffles and ganglioside (GM1(+)) lipid mediator release. Co-stimulation resulted in phalloidin-positive cytoneme-like cellular extensions, also known as tunneling nanotubes, which originated at points of calcium accumulation. This is the first report of cytoneme formation by mast cells. To determine the importance of lipid rafts for mast cell function, the cells were cholesterol depleted. Cholesterol depletion enhanced degranulation in resting, sensitized and co-stimulated cells, but not in FcepsilonRI-cross-linked cells, and inhibited formation of filamentous actin(+) cytonemes but not GM1(+) cytonemes. Treatment with latrunculin A to sequester globular-actin abolished cytoneme formation. The cytonemes may participate in intercellular communication during allergic and inflammatory responses, and their presence in the co-stimulated mast cells suggests new roles for CCRs in immunopathology. |
| Databáze: | OpenAIRE |
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