Preparation of polymer microspheres capable for pioglitazone release to modify macrophages function
| Autor: | Yasuhiko Tabata, Jun-ichiro Jo, Naoki Momotori |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
UV ultra violet PGA poly(glycolic acid) Pharmacology chemistry.chemical_compound PCR polymerase chain reaction 0302 clinical medicine Macrophage M-CSF macrophage colony stimulating factor lcsh:R5-920 TNF tumor necrosis factor lcsh:Cytology Chemistry BMDM mouse bone marrow derived-macrophage PLGA Interleukin ELISA enzyme-linked immunosorbent assay Microspheres PBS phosphate buffered-saline solution Arginase HPLC high performance liquid chromatography Drug delivery iNOS inducible nitric oxide synthase Original Article DDW double-distilled water medicine.symptom lcsh:Medicine (General) PLA poly(l-lactic acid) medicine.drug pio-MS PLGA microspheres incorporating pioglitazone Drug delivery system Biomedical Engineering Inflammation Biomaterials RS resulting solution 03 medical and health sciences FBS fetal bovine serum medicine SEM scanning electron microscopy Secretion lcsh:QH573-671 Pioglitazone Macrophages IMDM Iscove's modified Dulbecco's medium IL interleukin PPARγ peroxisome proliferator-activated receptor γ 030104 developmental biology PLGA poly(L-lactic-co-glycolic acid) SD standard deviation Poly(L-lactic-co-glycolic acid) 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Regenerative Therapy Regenerative Therapy, Vol 11, Iss, Pp 131-138 (2019) |
| ISSN: | 2352-3204 |
| Popis: | Introduction Macrophages play an important role in regulating inflammation and tissue regeneration. It is known that anti-inflammatory macrophages play an important role for tissue regeneration. The objective of this study is to modify macrophages phenotypes for anti-inflammatory function by utilizing drug delivery technology. Method In this study, 4 types of poly (L-lactic-co-glycolic acid) (PLGA) microspheres incorporating pioglitazone of an anti-inflammatory modifier (pio-MS) with different sizes were prepared. In vitro release test of pio-MS was performed in phosphate buffered-saline solution (PBS) containing 1 wt% of sodium lauryl sulfate. The arginase activity and the secretion of interleukin (IL)−10 as anti-inflammatory macrophage markers of mouse bone marrow derived-macrophages (BMDM) cultured with the pio-MS were evaluated. Results The sustained release of pioglitazone was observed from all types of pio-MS in vitro. When BMDM were cultured with the pio-MS with an average diameter of 40 μm (pio-MS40), the arginase activity and the secretion of IL-10 increased to a significant extent compared with other pio-MS. Conclusions The pio-MS40 with an diameter of 40 μm had a potential to induce the anti-inflammatory modification of BMDM in this culture system. The sustained release of pioglitazone is promoting to modify the macrophage function. Highlights • Microspheres incorporating pioglitazone with different sizes were prepared. • Sustained release of pioglitazone from the microspheres were observed. • The effect of pioglitazone on macrophages was enhanced by the sustained release. |
| Databáze: | OpenAIRE |
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