Non-invasive quantification of the mitochondrial redox state in livers during machine perfusion

Autor: Thomas M. van Gulik, Siavash Raigani, Korkut Uygun, Peili Chen, Stephanie E.J. Cronin, Reinier J. de Vries, Padraic Romfh, Benjamin T. Wilks, Shannon N. Tessier, Rohil Jain, Casie A. Pendexter, Heidi Yeh
Přispěvatelé: Graduate School, Surgery, Amsterdam Gastroenterology Endocrinology Metabolism
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Pathology
Physiology
Fulminant
medicine.medical_treatment
Mitochondria
Liver
Mitochondrion
Liver transplantation
Spectrum Analysis
Raman
Biochemistry
Vascular Medicine
Ischemia
Medicine and Health Sciences
Electrochemistry
Inner mitochondrial membrane
Energy-Producing Organelles
Multidisciplinary
Chemical Reactions
Mitochondria
Perfusion
Chemistry
Liver
Reperfusion Injury
Physical Sciences
Medicine
Cellular Structures and Organelles
Anatomy
Oxidation-Reduction
Research Article
Chemical Elements
medicine.medical_specialty
Science
Surgical and Invasive Medical Procedures
Bioenergetics
Digestive System Procedures
medicine
Animals
Humans
cardiovascular diseases
Transplantation
Machine perfusion
business.industry
Biology and Life Sciences
Biobehavioral Sciences
Cell Biology
Organ Transplantation
medicine.disease
Rats
Liver Transplantation
Oxygen
Early Diagnosis
Reperfusion
Mitochondrial Membrane
Physiological Processes
business
Reperfusion injury
Oxidation-Reduction Reactions
Zdroj: PLoS ONE, Vol 16, Iss 10, p e0258833 (2021)
PLoS ONE, 16(10 October):e0258833. Public Library of Science
PLoS ONE, Vol 16, Iss 10 (2021)
PLoS ONE
ISSN: 1932-6203
Popis: Ischemia reperfusion injury (IRI) is a critical problem in liver transplantation that can lead to life-threatening complications and substantially limit the utilization of livers for transplantation. However, because there are no early diagnostics available, fulminant injury may only become evident post-transplant. Mitochondria play a central role in IRI and are an ideal diagnostic target. During ischemia, changes in the mitochondrial redox state form the first link in the chain of events that lead to IRI. In this study we used resonance Raman spectroscopy to provide a rapid, non-invasive, and label-free diagnostic for quantification of the hepatic mitochondrial redox status. We show this diagnostic can be used to significantly distinguish transplantable versus non-transplantable ischemically injured rat livers during oxygenated machine perfusion and demonstrate spatial differences in the response of mitochondrial redox to ischemia reperfusion. This novel diagnostic may be used in the future to predict the viability of human livers for transplantation and as a tool to better understand the mechanisms of hepatic IRI.
Databáze: OpenAIRE
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