An immunoassay for human D dimer using monoclonal antibodies
| Autor: | D. Wyatt, Paul P. Masci, D.B. Rylatt, A.S. Blake, I H Bunce, A.N. Whitaker, M J Elms, W.A. Fletcher, D.A. Massingham, P.G. Bundesen, L.E. Cottis, A.J. Webber |
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| Rok vydání: | 1983 |
| Předmět: |
medicine.drug_class
Cross Reactions Monoclonal antibody Fibrinogen Epitope Fibrin Fibrinogen Degradation Products Immunoenzyme Techniques Mice Antigen Antibody Specificity D-dimer medicine Animals Humans Fibrinogen degradation product biology medicine.diagnostic_test Chemistry Fibrinolysis Antibodies Monoclonal Hematology Disseminated Intravascular Coagulation Molecular biology Biochemistry Immunoassay biology.protein Antibody medicine.drug |
| Zdroj: | Thrombosis Research. 31:767-778 |
| ISSN: | 0049-3848 |
| DOI: | 10.1016/0049-3848(83)90108-1 |
| Popis: | Monoclonal antibodies (MAb) were raised against human D dimer. The hybridomas were screened with a solid phase enzyme immunoassay against D dimer and fibrinogen degradation products. Among the panel of MAb identified, two distinct patterns emerged; the majority belonging to a panspecific class reacting against epitopes present on both D dimer and fibrinogen degradation product Dcate and a monospecific class reacting with determinants apparently present only on D dimer. A number of MAb were further characterised for their ability to specifically capture antigen in a solid phase enzyme immunoassay and assays were developed which have a sensitivity of 10 ng/ml for D dimer or crosslinked fibrin derivatives and may be suitable for detection of crosslinked derivatives in serum and plasma samples in a clinical situation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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