Effect of valine and the herbicide sulfometuron methyl on acetolactate synthase activity in nuclear and plasmid-borne sulphometuron methyl resistant Saccharomyces cerevisiae strains
| Autor: | G. H. Rank, S. N. Maiti, M. W. Zink |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Acetolactate synthase activity
Immunology Saccharomyces cerevisiae Biology Applied Microbiology and Biotechnology Microbiology Transformation Genetic Plasmid Valine Genetics Allele Molecular Biology Gene Alleles Cell Nucleus Acetolactate synthase Herbicides Oxo-Acid-Lyases Drug Resistance Microbial General Medicine biology.organism_classification Culture Media Acetolactate Synthase Sulfonylurea Compounds Biochemistry Mutation biology.protein Specific activity Plasmids |
| Zdroj: | Canadian Journal of Microbiology. 34:680-685 |
| ISSN: | 1480-3275 0008-4166 |
| DOI: | 10.1139/m88-112 |
| Popis: | Acetolactate synthase (ALS) specific activity was evaluated in isogenic lines of Saccharomyces cerevisiae carrying the wild-type ILV2 gene or mutations in this gene for resistance to the herbicide sulfometuron methyl (SM). Statistical comparisons were made between two nuclear alleles and among five alleles borne on a YE chimaeric plasmid transformed into a strain carrying a 1.5-kilobase deletion in the nuclear ILV2 gene. Decreased ALS activity of plasmid-borne SM-resistant mutations was shown not to be caused by copy number effects. ALS-specific activity in strains carrying the wild-type ILV2 allele exhibited strong feedback inhibition by valine and was sensitive to SM. All nuclear and plasmid-borne SM-resistance alleles resulted in ALS-specific activity highly resistant to SM and resistant to valine feedback inhibition. |
| Databáze: | OpenAIRE |
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