Escherichia coli limits Salmonella Typhimurium infections after diet shifts and fat-mediated microbiota perturbation in mice
| Autor: | Michael B. Zimmermann, Uwe Sauer, Alexander O. Brachmann, Erik Bakkeren, Bidong D. Nguyen, Bärbel Stecher, Markus Beutler, Markus Arnoldini, Wolf-Dietrich Hardt, Shinichi Sunagawa, Lisa A. Maier, Ersin Gül, Daniel Hoces, Athanasios Typas, Médéric Diard, Tamas Dolowschiak, Andrew J. Macpherson, Markus Kreuzer, Annika Hausmann, Sandra Y. Wotzka, Mirjam Zünd, Jörn Piel, Dorothée L. Berthold, Kathrin Moor, Tobias Fuhrer, Emma Slack |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Salmonella typhimurium Microbiology (medical) Salmonella Animal feed Immunology Oleic Acids Colonisation resistance Biology medicine.disease_cause digestive system Applied Microbiology and Biotechnology Microbiology Article Bile Acids and Salts Mice 03 medical and health sciences chemistry.chemical_compound Plasmid Escherichia coli Genetics medicine Animals Microbiome Pathogen 030304 developmental biology 2. Zero hunger 0303 health sciences 030306 microbiology Cell Biology Animal Feed Dietary Fats Gastrointestinal Microbiome 3. Good health Mice Inbred C57BL Oleic acid chemistry Host-Pathogen Interactions Microbial Interactions Female |
| Zdroj: | Nature Microbiology, 4 (12) Nature microbiology |
| ISSN: | 2058-5276 |
| Popis: | The microbiota confers colonization resistance, which blocks Salmonella gut colonization1. As diet affects microbiota composition, we studied whether food composition shifts enhance susceptibility to infection. Shifting mice to diets with reduced fibre or elevated fat content for 24 h boosted Salmonella Typhimurium or Escherichia coli gut colonization and plasmid transfer. Here, we studied the effect of dietary fat. Colonization resistance was restored within 48 h of return to maintenance diet. Salmonella gut colonization was also boosted by two oral doses of oleic acid or bile salts. These pathogen blooms required Salmonella’s AcrAB/TolC-dependent bile resistance. Our data indicate that fat-elicited bile promoted Salmonella gut colonization. Both E. coli and Salmonella show much higher bile resistance than the microbiota. Correspondingly, competitive E. coli can be protective in the fat-challenged gut. Diet shifts and fat-elicited bile promote S. Typhimurium gut infections in mice lacking E. coli in their microbiota. This mouse model may be useful for studying pathogen–microbiota–host interactions, the protective effect of E. coli, to analyse the spread of resistance plasmids and assess the impact of food components on the infection process. Short-term exposure to a high-fat diet reduces colonization resistance to Salmonella Typhimurium infection in mice and is associated with increase bile salts and plasmid transfer; however, E. coli can provide a protective effect under these conditions. |
| Databáze: | OpenAIRE |
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