Characterizing a novel and sensitive method to measure dsRNA in soil

Autor: Fatima Zapata, Samuel Dubelman, Steven L. Levine, Peter D. Jensen, Joshua R. Fischer, Geoffrey M. Mueller
Rok vydání: 2016
Předmět:
Zdroj: Chemosphere. 161:319-324
ISSN: 0045-6535
Popis: Performing environmental assessments for double-stranded RNA-based agricultural products require the development of sensitive and selective methods to measure biodegradation rates of dsRNAs. We developed and characterized a novel analytical procedure that uses a molecular hybridization assay (QuantiGene®) to accurately measure dsRNA extracted from diverse soils. In this report, we utilize this method to demonstrate that two dsRNAs with distinct size, structure, and sequence degrade rapidly in soil with indistinguishable kinetics.
Databáze: OpenAIRE