Small extracellular vesicles from human adipose‐derived stem cells attenuate cartilage degeneration
| Autor: | Ye Eun Yun, Youn Jae Jung, Dong-Gyu Jo, Ji Suk Choi, Chang Hee Woo, Siyoung Yang, Gun Young Jung, Jae Hyung Park, Yong Woo Cho, Jeongmi Lee, Woo Sung Kim, Jihoon Han, Hark Kyun Kim, Kyoung-Soo Lee |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Histology Adipose tissue Osteoarthritis Matrix (biology) Chondrocyte Flow cytometry 03 medical and health sciences 0302 clinical medicine medicine lcsh:QH573-671 medicine.diagnostic_test lcsh:Cytology Chemistry Cartilage immune regulation Mesenchymal stem cell Cell Biology medicine.disease Cell biology osteoarthritis 030104 developmental biology medicine.anatomical_structure human adipose-derived stem cells 030220 oncology & carcinogenesis Stem cell extracellular vesicles Research Article |
| Zdroj: | Journal of Extracellular Vesicles, Vol 9, Iss 1 (2020) Journal of Extracellular Vesicles |
| ISSN: | 2001-3078 |
| Popis: | Osteoarthritis (OA) is a chronic degenerative disease of articular cartilage that is the most common joint disease worldwide. Mesenchymal stem cells (MSCs) have been the most extensively explored for the treatment of OA. Recently, it has been demonstrated that MSC-derived extracellular vesicles (EVs) may contribute to the potential mechanisms of MSC-based therapies. In this study, we investigated the therapeutic potential of human adipose-derived stem cells EVs (hASC-EVs) in alleviating OA, along with the mechanism. EVs were isolated from the culture supernatants of hASCs by a multi-filtration system based on the tangential flow filtration (TFF) system. The isolated EVs were characterised using dynamic light scattering (DLS), transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA) and flow cytometry analysis. The hASC-EVs not only promoted the proliferation and migration of human OA chondrocytes, but also maintained the chondrocyte matrix by increasing type Ⅱ collagen synthesis and decreasing MMP-1, MMP-3, MMP-13 and ADAMTS-5 expression in the presence of IL-1β in vitro. Intra-articular injection of hASC-EVs significantly attenuated OA progression and protected cartilage from degeneration in both the monosodium iodoacetate (MIA) rat and the surgical destabilisation of the medial meniscus (DMM) mouse models. In addition, administration of hASC-EVs inhibited the infiltration of M1 macrophages into the synovium. Overall results suggest that the hASC-EVs should be considered as a potential therapeutic approach in the treatment of OA. |
| Databáze: | OpenAIRE |
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