Evidence that agricultural use of pesticides selects pyrethroid resistance within Anopheles gambiae s.l. populations from cotton growing areas in Burkina Faso, West Africa

Autor: Moussa Namountougou, Abdoulaye Diabaté, Dieudonné Diloma Soma, Thierry Baldet, Aristide Sawdetuo Hien, Omer S.A. Hema, Bazoma Bayili, Olivier Gnankiné, Kounbobr Roch Dabiré
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Identification
Veterinary medicine
Insecticides
Life Cycles
Anopheles gambiae
Anopheles Gambiae
Drug Resistance
lcsh:Medicine
H02 - Pesticides
Cotton
Disease Vectors
L73 - Maladies des animaux
Polymerase Chain Reaction
Mosquitoes
Insecticide Resistance
chemistry.chemical_compound
0302 clinical medicine
Larvae
Pyrethrins
Medicine and Health Sciences
lcsh:Science
Flowering Plants
Larva
Résistance aux insecticides
Multidisciplinary
Agriculture
Plants
Insects
Pratique culturale
Africa
Western
PCR
Vecteur de maladie
Infectious Diseases
Agrochemicals
L72 - Organismes nuisibles des animaux
Research Article
Enquête organismes nuisibles
Arthropoda
Death Rates
030231 tropical medicine
Context (language use)
Biology
Pollution par l'agriculture
03 medical and health sciences
Surface Water
Anopheles
Burkina Faso
parasitic diseases
Animals
Pesticides
Demography
Gossypium
Pesticide residue
business.industry
lcsh:R
Organisms
Biology and Life Sciences
Pesticide
biology.organism_classification
Résidu de pesticide
Invertebrates
Biotechnology
Insect Vectors
Pyréthrine
Species Interactions
030104 developmental biology
Deltamethrin
chemistry
Gène
Vector (epidemiology)
Mutation
People and Places
Earth Sciences
lcsh:Q
Pest Control
Hydrology
business
Developmental Biology
Zdroj: PLoS ONE, Vol 12, Iss 3, p e0173098 (2017)
PLoS ONE
PloS One
ISSN: 1932-6203
Popis: Many studies have shown the role of agriculture in the selection and spread of resistance of Anopheles gambiae s.l. to insecticides. However, no study has directly demonstrated the presence of insecticides in breeding sources as a source of selection for this resistance. It is in this context that we investigated the presence of pesticide residues in breeding habitats and their formal involvement in vector resistance to insecticides in areas of West Africa with intensive farming. This study was carried out from June to November 2013 in Dano, southwest Burkina Faso in areas of conventional (CC) and biological cotton (BC) growing. Water and sediment samples collected from breeding sites located near BC and CC fields were submitted for chromatographic analysis to research and titrate the residual insecticide content found there. Larvae were also collected in these breeding sites and used in toxicity tests to compare their mortality to those of the susceptible strain, Anopheles gambiae Kisumu. All tested mosquitoes (living and dead) were analyzed by PCR for species identification and characterization of resistance genes. The toxicity analysis of water from breeding sites showed significantly lower mortality rates in breeding site water from biological cotton (WBC) growing sites compared to that from conventional cotton (WCC) sites respective to both An. gambiae Kisumu (WBC: 80.75% vs WCC: 92.75%) and a wild-type strain (49.75% vs 66.5%). The allele frequencies L1014F, L1014S kdr, and G116S ace -1R mutations conferring resistance, respectively, to pyrethroids and carbamates / organophosphates were 0.95, 0.4 and 0.12. Deltamethrin and lambda-cyhalothrin were identified in the water samples taken in October/November from mosquitoes breeding in the CC growing area. The concentrations obtained were respectively 0.0147ug/L and 1.49 ug/L to deltamethrin and lambdacyhalothrin. Our results provided evidence by direct analysis (biological and chromatographic tests) of the role of agriculture as a source of selection pressure on vectors to insecticides used in growing areas.
Databáze: OpenAIRE
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