Production of hepatitis B virion-like particles in yeast
| Autor: | Shiosaki Kouichi, Nishiinura Shin-ichiro, Mizokami Hiroshi, Takata Ken-ichi, Matsubara Kenichi |
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| Rok vydání: | 1991 |
| Předmět: |
Hepatitis B virus
Antigenicity Lysis Octoxynol Blotting Western Saccharomyces cerevisiae Biology Virus Replication Polyethylene Glycols law.invention law Centrifugation Density Gradient Genetics Promoter Regions Genetic Hepatitis B Surface Antigens Glyceraldehyde-3-Phosphate Dehydrogenases General Medicine biology.organism_classification Hepatitis B Core Antigens Molecular biology Yeast Microscopy Electron Terminator (genetics) Capsid Hepadnaviridae Recombinant DNA Plasmids |
| Zdroj: | Gene. 106:143-149 |
| ISSN: | 0378-1119 |
| Popis: | We constructed a yeast strain that simultaneously expresses four genes encoding the major S, middle S, large S hepatitis B viral envelope proteins and the core protein under the control of the yeast glyceraldehyde-3-phosphate dehydrogenase promoter and terminator. The lysate from this cell line, examined by immunological, physicochemical methods and electron microscopy, was found to contain spherical particles with a diameter of about 40 nm and a density of 1.25g/ml. These particles reacted with anti-envelope antibodies, but not with anti-core antibodies. However, core antigenicity appeared upon treatment with 3 % Nonidet P-40 that eliminates an outer envelope. These observations suggest production of a virion-like complex structure, or at least its DNA-less analog, consisting of core particle enveloped by antibody-reactive envelope. Such a structure was made only when all the four gene products were synthesized in a yeast cell. This system may be useful for the study of virus structure and assembly, and for improved vaccine development. |
| Databáze: | OpenAIRE |
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