Enzymatic, metabolic and secretory patterns in human islets of type 2 (non-insulin-dependent) diabetic patients
| Autor: | Ramon Gomis, Josefa Fernandez-Álvarez, Abdullah Sener, Willy Malaisse, Joanne Rasschaert, Ignacio Conget |
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| Rok vydání: | 1994 |
| Předmět: |
Adult
Male medicine.medical_specialty Adolescent Glycerol phosphate shuttle Endocrinology Diabetes and Metabolism medicine.medical_treatment Glutamate decarboxylase Glycerolphosphate Dehydrogenase Biology Transaminase chemistry.chemical_compound Islets of Langerhans Glutamate Dehydrogenase Reference Values Internal medicine Hexokinase Glucokinase Insulin Secretion Internal Medicine medicine Humans Insulin Aspartate Aminotransferases Aged Glutamate Decarboxylase Pancreatic islets Glutamate dehydrogenase Alanine Transaminase Middle Aged Endocrinology medicine.anatomical_structure Glucose chemistry Diabetes Mellitus Type 2 Female Glycolysis |
| Zdroj: | Diabetologia. 37(2) |
| ISSN: | 0012-186X |
| Popis: | Islets were isolated by automatic digestion from non-diabetic cadaveric organ donors and from Type 2 (non-insulin-dependent) diabetic subjects. The activity of FAD-glycerophosphate dehydrogenase, but not that of either glutamate dehydrogenase, glutamate-oxalacetate transaminase or glutamate-pyruvate transaminase, was lower in Type 2 diabetic patients than control subjects. Hexokinase, glucokinase and glutamate decarboxylase activities were also measured in islets from control subjects. The utilization of D-[5-3H]glucose, oxidation of D-[6-14C]glucose and release of insulin evoked by D-glucose were all lower in Type 2 diabetic patients than control subjects. The secretory response to the combination of L-leucine and L-glutamine appeared less severely affected. Islets from Type 2 diabetic patients may thus display enzymatic, metabolic and secretory anomalies similar to those often observed in animal models of Type 2 diabetes, including a deficiency of beta-cell FAD-linked glycerophosphate dehydrogenase, the key enzyme of the glycerol phosphate shuttle. |
| Databáze: | OpenAIRE |
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