Effective characterization of Salmonella Enteritidis by most probable number (MPN) followed by multiplex polymerase chain reaction (PCR) methods
| Autor: | Maria Tereza Pepe Razzolini, Maria Helena Matté, Lincohn Zappelini, Solange Martone-Rocha, Monique Ribeiro Tiba, Milena Dropa, Bruna Suellen Breternitz |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Serotype Salmonella Health Toxicology and Mutagenesis Salmonella enteritidis 030106 microbiology Pcr assay Sewage Biology medicine.disease_cause Microbiology 03 medical and health sciences Most probable number Multiplex polymerase chain reaction medicine Environmental Chemistry Serotyping Pathogen Probability business.industry General Medicine Pollution Virology 030104 developmental biology ÁGUA POTÁVEL business Multiplex Polymerase Chain Reaction |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1614-7499 |
| Popis: | Nontyphoidal Salmonella (NTS) is a relevant pathogen involved in gastroenteritis outbreaks worldwide. In this study, we determined the capacity to combine the most probable number (MPN) and multiplex polymerase chain reaction (PCR) methods to characterize the most important Salmonella serotypes in raw sewage. A total of 499 isolates were recovered from 27 raw sewage samples and screened using two previously described multiplex PCR methods. From those, 123 isolates were selected based on PCR banding pattern—identical or similar to Salmonella Enteritidis and Salmonella Typhimurium—and submitted to conventional serotyping. Results showed that both PCR assays correctly serotyped Salmonella Enteritidis, however, they presented ambiguous results for Salmonella Typhimurium identification. These data highlight that MPN and multiplex PCR can be useful methods to describe microbial quality in raw sewage and suggest two new PCR patterns for Salmonella Enteritidis identification. |
| Databáze: | OpenAIRE |
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