Relation between T cell number and epithelial HLA class II expression quantified by image analysis in normal and inflamed human gastric mucosa
| Autor: | K Valnes, Per Brandtzaeg, H S Huitfeldt |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Pathology
medicine.medical_specialty T cell T-Lymphocytes Chronic gastritis Fluorescent Antibody Technique Human leukocyte antigen Biology Epithelium Leukocyte Count Antigen Reference Values medicine Gastric mucosa Image Processing Computer-Assisted Humans Lamina propria HLA-D Antigens Gastroenterology HLA-DR Antigens medicine.disease Foveolar cell medicine.anatomical_structure Gastric Mucosa Gastritis medicine.symptom Research Article |
| Zdroj: | Gut. 31(6) |
| ISSN: | 0017-5749 |
| Popis: | Epithelial expression of HLA class II determinants and the number of lamina propria and intraepithelial T cells were quantified in gastric body mucosa by means of paired immunofluorescence staining which was subjected to computerised image analysis. In normal mucosa, epithelial HLA-DR expression was virtually absent. A significantly increased expression was seen in simple chronic gastritis, most extensively in the isthmus zone, where a positive reaction was seen in 34% of the epithelial area when the gastritis was of low degree and in 85% when it was of moderate severity. The most extensive HLA-DR expression was found in moderate 'stump gastritis' 28 to 32 years after Billroth II resections. In these patients the epithelial area in the foveolar and isthmus zones showed 83% and 92% positive responses, respectively. The HLA subregion products were expressed in a differential manner (DR greater than DP greater than DQ). The number of both intraepithelial and lamina propria T cells increased significantly with increasing severity of gastritis, and the fraction of putative memory T cells was also raised. Correlation analyses showed a positive relation between the epithelial expression of HLA-DR and the intraepithelial as well as the lamina propria density of T cell. These results suggest a biological link between T cells, aberrant HLA-DR expression, and gastritis, although the pathogenic importance of this relation is unknown. Enhanced epithelial presentation of autoantigens or luminal antigens, or both leading to increased activation of T cells is one possible explanation. |
| Databáze: | OpenAIRE |
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