Characterization of an alternative splice variant of human nucleoside triphosphate diphosphohydrolase 3 (NTPDase3): A possible modulator of nucleotidase activity and purinergic signaling
| Autor: | Terence L. Kirley, Patrick A. Crawford, Thomas M. Smith, Keith J. Gaddie |
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| Rok vydání: | 2007 |
| Předmět: |
Nucleotidase activity
Molecular Sequence Data Biophysics Biology Biochemistry Article Enzyme activator chemistry.chemical_compound Exon Chlorocebus aethiops Animals Humans Amino Acid Sequence Molecular Biology Gene Library Adenosine Triphosphatases COS cells Base Sequence Apyrase Cell Membrane Alternative splicing Molecular biology Protein Structure Tertiary Enzyme Activation Alternative Splicing Protein Transport Transmembrane domain chemistry COS Cells Nucleoside triphosphate Signal Transduction |
| Zdroj: | Archives of Biochemistry and Biophysics. 457:7-15 |
| ISSN: | 0003-9861 |
| DOI: | 10.1016/j.abb.2006.10.013 |
| Popis: | Nucleoside triphosphate diphosphohydrolase 3 (NTPDase3) is a cell surface, membrane-bound enzyme that hydrolyzes extracellular nucleotides, thereby modulating purinergic signaling. An alternatively spliced variant of NTPDase3 was obtained and analyzed. This alternatively spliced variant, termed "NTPDase3beta", is produced through the use of an alternative terminal exon (exon 11) in place of the terminal exon (exon 12) in the full-length NTPDase3, now termed "NTPDase3alpha". This results in an expressed protein lacking the C-terminal cytoplasmic sequence, the C-terminal transmembrane helix, and apyrase conserved region 5. The cDNA encoding this truncated splice variant was detected in a human lung library by PCR. Like the full-length NTPDase3alpha, the alternatively spliced NTPDase3beta was expressed in COS cells after transfection, but only the full-length NTPDase3alpha is enzymatically active and properly trafficked to the plasma membrane. However, when the truncated NTPDase3beta was co-transfected with full-length NTPDase3alpha, there was a significant reduction in the amount of NTPDase3alpha that was properly processed and trafficked to the plasma membrane as active enzyme, indicating that the truncated form interferes with normal biosynthetic processing of the full-length enzyme. This suggests a role for the NTPDase3beta variant in the regulation of NTPDase3 nucleotidase activity, and therefore the control of purinergic signaling, in those cells and tissues expressing both NTPDase3alpha and NTPDase3beta. |
| Databáze: | OpenAIRE |
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