Participation of the overproduced elongation factor Tu from Thermus thermophilus in protein biosynthesis of Escherichia coli
| Autor: | Waltraud Zeidler, Mathias Sprinzl, Roland Kreutzer |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Peptide Biosynthesis
tuf-gene expression Pyridones Biophysics Gene Expression Peptide Elongation Factor Tu medicine.disease_cause environment and public health Biochemistry Plasmid Bacterial Proteins Kirromycin GTPase activity Structural Biology Genetics Protein biosynthesis medicine Escherichia coli Polysome block Molecular Biology chemistry.chemical_classification biology Thermus thermophilus food and beverages Drug Resistance Microbial Cell Biology biology.organism_classification Enterobacteriaceae enzymes and coenzymes (carbohydrates) Enzyme chemistry Protein Biosynthesis bacteria Transformation Bacterial Peptides Ribosomes EF-Tu Bacteria Plasmids |
| Zdroj: | FEBS letters. 319(1-2) |
| ISSN: | 0014-5793 |
| Popis: | The influence of the overproduced elongation factor Tu (EF-Tu) from Thermus thermophilus on the protein biosynthesis in Escherichia coli was investigated both in vivo and in vitro. A kirromycin-resistant E. coli strain became sensitive to this antibiotic upon the expression of the tufA-gene of T. thermophilus present on a plasmid. In in vitro translation with components of the kirromycin-resistant E. coli strain the poly(Phe) synthesis stopped when minute amounts of the EF-Tu from T. thermophilus were added. Both results indicate the sensitivity of the T. thermophilus EF-Tu to kirromycin and its participation in the polypeptide synthesis of E. coli. |
| Databáze: | OpenAIRE |
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