Small size fullerenol nanoparticles inhibit thrombosis and blood coagulation through inhibiting activities of thrombin and FXa
| Autor: | Xing Gengyan, Weihong Gu, Yanan Chang, Gengmei Xing, Kui Chen, Juan Li, Jinke Liu, Shibo Xia, Li Zeng, Xue Bai, Jinxia Li, Mian Zu, Lina Zhao |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Bioengineering 02 engineering and technology Pharmacology Rats Sprague-Dawley 03 medical and health sciences Thrombin In vivo medicine Animals General Materials Science Thrombus Blood Coagulation Prothrombin time medicine.diagnostic_test Chemistry Coronary Thrombosis Anticoagulants 021001 nanoscience & nanotechnology medicine.disease Thrombosis In vitro Rats 030104 developmental biology Coagulation Factor Xa Molecular Medicine Nanoparticles Fullerenes 0210 nano-technology medicine.drug Partial thromboplastin time |
| Zdroj: | Nanomedicine : nanotechnology, biology, and medicine. 14(3) |
| ISSN: | 1549-9642 |
| Popis: | Thrombus is one of main causes of death in the world and also a vital trouble of biomaterials application in vivo. Recently, effect of fullerenol nanomaterials on anticoagulation was found in our research through extension of bleeding times in treated Sprague-Dawley rats via intravenous injection. Inhibiting of fullerenols on thrombosis was ascertained further by thromboembolism model. Effects of fullerenols on intrinsic and extrinsic pathway were distinct in prolonging activated partial thromboplastin time and prothrombin time, which supported that fullerenols induced defects in both pathways. Inhibited activities of activated coagulation factor X (FXa) and thrombin were verified by experiments in vitro and AutoDock Vina. The results suggest that fullerenols depending on small size and certainly surface property occupied the active domain of FXa and thrombin to block their activity; further, thrombosis was inhibited. This putative mechanism offers an insight into how fullerenol NPs were utilized further in biomedical applications. |
| Databáze: | OpenAIRE |
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