Pedigreed Primate Embryonic Stem Cells Express Homogeneous Familial Gene Profiles
| Autor: | Meena Sukhwani, Carlos A. Castro, Kyle E. Orwig, Jocelyn D. Mich-Basso, Christopher S. Navara, Elizabeta Kovkarova-Naumovski, Ahmi Ben-Yehudah, Calvin Simerly, Gerald Schatten, Naftali Kaminski, Ethan Jacoby, Carrie J. Redinger |
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| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Male
Article biology.animal Gene expression Animals Primate Gene reproductive and urinary physiology Cells Cultured Embryonic Stem Cells Genetics biology Gene Expression Profiling Gene Expression Regulation Developmental Karyotype Embryo Cell Biology Embryonic stem cell Macaca mulatta Pedigree Gene expression profiling embryonic structures Molecular Medicine Female Ploidy biological phenomena cell phenomena and immunity Developmental Biology |
| Popis: | Human embryonic stem cells (hESCs) hold great biomedical promise, but experiments comparing them produce heterogeneous results, raising concerns regarding their reliability and utility, although these variations may result from their disparate and anonymous origins. To determine whether primate ESCs have intrinsic biological limitations compared with mouse ESCs, we examined expression profiles and pluripotency of newly established nonhuman primate ESC (nhpESCs). Ten pedigreed nhpESC lines, seven full siblings (fraternal quadruplets and fraternal triplets), and nine half siblings were derived from 41 rhesus embryos; derivation success correlated with embryo quality. Each line has been growing continuously for ∼1 year with stable diploid karyotype (except for one stable trisomy) and expresses in vitro pluripotency markers, and eight have already formed teratomas. Unlike the heterogeneous gene expression profiles found among hESCs, these nhpESCs display remarkably homogeneous profiles (>97%), with full-sibling lines nearly identical (>98.2%). Female nhpESCs express genes distinct from their brother lines; these sensitive analyses are enabled because of the very low background differences. Experimental comparisons among these primate ESCs may prove more reliable than currently available hESCs, since they are akin to inbred mouse strains in which genetic variables are also nearly eliminated. Finally, contrasting the biological similarities among these lines with the heterogeneous hESCs might suggest that additional, more uniform hESC lines are justified. Taken together, pedigreed primate ESCs display homogeneous and reliable expression profiles. These similarities to mouse ESCs suggest that heterogeneities found among hESCs likely result from their disparate origins rather than intrinsic biological limitations with primate embryonic stem cells. Disclosure of potential conflicts of interest is found at the end of this article. |
| Databáze: | OpenAIRE |
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