Cell free translation of chicken calcitonin messenger RNA
| Autor: | Gérard Milhaud, Annick Jullienne, Claude Desplan, F. Lasmoles, F. Treilhou-Lahille, N. Segond, M.S. Moukhtar |
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| Rok vydání: | 1983 |
| Předmět: |
Calcitonin
Translation Radioimmunoassay Biophysics Biochemistry chemistry.chemical_compound Biosynthesis Reticulocyte Structural Biology Genetics medicine Animals RNA Messenger Molecular Biology Polyacrylamide gel electrophoresis Messenger RNA Methionine Cell-Free System Chemistry Immunochemistry RNA Cell Biology Chicken medicine.anatomical_structure Protein Biosynthesis Electrophoresis Polyacrylamide Gel Ultimobranchial gland PMSF Poly A Chickens |
| Zdroj: | FEBS Letters. 157:100-104 |
| ISSN: | 0014-5793 |
| Popis: | The primary step of calcitonin biosynthesis was studied in a normal organ: chicken ultimobranchial gland, a tissue particularly rich in calcitonin secretory cells. Poly(A)-rich RNA was extracted and purified from ultimobranchial organs and translated in a reticulocyte lysate in the presence of labelled methionine. Polyacrylamide gel electrophoresis of specific immunoprecipitates revealed a major band of Mr 14 500 and a band of Mr 13 300. Thus, in chicken the precursor of calcitonin is a Mr 14500 polypeptide. The minor component of Mr 13 300 could represent limited processing by the reticulocyte lysate. |
| Databáze: | OpenAIRE |
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