Down-regulated miR-448 relieves spinal cord ischemia/reperfusion injury by up-regulating SIRT1

Autor: Wang Yun, Hai-Hao Wu, Dong-Ying Tao, Qing-Jiang Pang, Jiang-Tao Liu
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Male
0301 basic medicine
Physiology
Apoptosis
Endogeny
Pharmacology
Biochemistry
Rats
Sprague-Dawley
0302 clinical medicine
Sirtuin 1
General Pharmacology
Toxicology and Pharmaceutics
lcsh:QH301-705.5
Research Articles
lcsh:R5-920
medicine.diagnostic_test
Reverse Transcriptase Polymerase Chain Reaction
General Neuroscience
miR-448
General Medicine
Transfection
Flow Cytometry
Up-Regulation
Blot
Reperfusion Injury
medicine.symptom
lcsh:Medicine (General)
SCII
animal structures
Blotting
Western
Immunology
Biophysics
Nerve cells
Down-Regulation
Ocean Engineering
Flow cytometry
03 medical and health sciences
SIRT1
Western blot
medicine
Animals
Spinal Cord Ischemia
business.industry
Cell Biology
Hypoxia (medical)
medicine.disease
Rats
Disease Models
Animal
MicroRNAs
030104 developmental biology
lcsh:Biology (General)
business
Reperfusion injury
030217 neurology & neurosurgery
Zdroj: Brazilian Journal of Medical and Biological Research, Volume: 51, Issue: 5, Article number: e7319, Published: 15 MAR 2018
Brazilian Journal of Medical and Biological Research, Vol 51, Iss 5 (2018)
Brazilian Journal of Medical and Biological Research v.51 n.5 2018
Brazilian Journal of Medical and Biological Research
Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
Popis: MicroRNAs play a crucial role in the progression of spinal cord ischemia/reperfusion injury (SCII). The role of miR-448 and SIRT1 in SCII was investigated in this study, to provide further insights into prevention and improvement of this disorder. In this study, expressions of miR-448 and SIRT1 protein were determined by qRT-PCR and western blot, respectively. Flow cytometry was used to analyze cell apoptosis. The endogenous expression of genes was modulated by recombinant plasmids and cell transfection. Dual-luciferase reporter assay was performed to determine the interaction between miR-448 and SIRT1. The Basso, Beattie, and Bresnahan score was used to measure the hind-limb function of rat. The spinal cord ischemia reperfusion injury model of adult rats was developed by abdominal aorta clamping, and the nerve function evaluation was completed by motor deficit index score. In SCII tissues and cells treated with hypoxia, miR-448 was up-regulated while SIRT1 was down-regulated. Hypoxia treatment reduced the expression of SIRT1 through up-regulating miR-448 in nerve cells. Up-regulation of miR-448 induced by hypoxia promoted apoptosis of nerve cells through down-regulating SIRT1. Down-regulated miR-448 improved neurological function and hind-limb motor function of rats with SCII by up-regulating SIRT1. Down-regulated miR-448 inhibited apoptosis of nerve cells and improved neurological function by up-regulating SIRT1, which contributes to relieving SCII.
Databáze: OpenAIRE
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