Corneal thickness and endothelial cell density measured by non-contact specular microscopy and pachymetry in Rhesus macaques (Macaca mulatta) with laser-induced ocular hypertension
| Autor: | Maria E. Kallberg, F. J. Ollivier, H.L. Sapp, Mark B. Sherwood, Dennis Brooks, Stacy E. Andrew, William W. Dawson, András M. Komáromy |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Intraocular pressure
Corneal endothelium medicine.medical_specialty Time Factors genetic structures Endothelium Glaucoma Ocular hypertension Cell Count Cornea Cellular and Molecular Neuroscience Ophthalmology medicine Animals Cell Size Microscopy business.industry Lasers Endothelium Corneal Ketamine hydrochloride Anatomy medicine.disease Macaca mulatta eye diseases Sensory Systems medicine.anatomical_structure Models Animal SPECULAR MICROSCOPY Ocular Hypertension sense organs business |
| Zdroj: | Experimental Eye Research. 76:671-677 |
| ISSN: | 0014-4835 |
| DOI: | 10.1016/s0014-4835(03)00055-1 |
| Popis: | PURPOSE Sustained increase in intraocular pressure (IOP) in humans results in a loss of corneal endothelial cells and an increase of corneal thickness. The effects of chronically elevated IOP on the corneal endothelium of monkeys with laser-induced ocular hypertension, a commonly used animal model of human glaucoma have not been documented. This study examined the central corneal thickness (CCT), the corneal endothelial cell density (ECD), and the corneal endothelial cell size (ACS) in Rhesus monkeys with experimental ocular hypertension. Materials and methods. Ten male monkeys with argon laser-induced ocular hypertension in one eye for an average duration of 2.4+/-0.7 years, were sedated with ketamine hydrochloride, and the CCT, ECD, and ACS measured at the center of the cornea of both eyes with a Topcon SP-2000P non-contact specular microscope (Topcon America Corporation((R)), Paramus, NJ, USA). CCT was also measured using a DHG-500 Pachette ultrasonic pachymeter (DHG Technology Inc., Exton, PA, USA). Mean and standard deviation (S.D.) of CCT, ECD and ACS for each eye was calculated and statistically compared.Results. Mean CCT in the hypertensive and normal eyes measured by specular microscopy was 0.477+/-0.023mm and 0.468+/-0.020 mm, respectively. Mean ECD in the hypertensive and normal eyes was 2601.7+/-631.8 and 3990.2+/-402.9 cells mm(-2), respectively. The mean size of the endothelial cells was 252.4+/-23.9 micro m(2) in the normal eye and 408.7+/-115.0 microm m(2) in the hypertensive eye. No significant difference in the measurement of CCT was observed between the specular microscope and the pachymeter (p=0.46). No significant difference in the mean CCT was observed between the two eyes (p=0.4820), whereas the mean ECD was significantly lower in the hypertensive eye than in the normal eye (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect