Protective effects of zinc salts on TPA-induced hepatic and brain lipid peroxidation, glutathione depletion, DNA damage and peritoneal macrophage activation in mice
| Autor: | Philip J. Vuchetich, Sidhartha D. Ray, M. X. Tran, R. L. Krohn, Sidney J. Stohs, Manashi Bagchi, Debasis Bagchi |
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| Rok vydání: | 1998 |
| Předmět: |
Antioxidant
DNA damage medicine.medical_treatment chemistry.chemical_element Tocopherols Cytochrome c Group Mitochondria Liver Zinc medicine.disease_cause Gluconates Lipid peroxidation chemistry.chemical_compound Mice Methionine medicine Organometallic Compounds Animals Vitamin E Drug Interactions Pharmacology chemistry.chemical_classification Reactive oxygen species Brain Glutathione Macrophage Activation Molecular biology Zinc Sulfate chemistry Biochemistry Liver Zinc Compounds Luminescent Measurements Macrophages Peritoneal Tetradecanoylphorbol Acetate Female Lipid Peroxidation Oxidation-Reduction Oxidative stress DNA Damage |
| Zdroj: | General pharmacology. 30(1) |
| ISSN: | 0306-3623 |
| Popis: | 1. 1. The comparative protective abilities of zinc l -methionine, zinc dl -methionine, zinc sulfate, zinc gluconate, l -methionine, dl -methionine, and vitamin E succinate (VES) on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced lipid peroxidation, DNA fragmentation, and glutathione depletion in the hepatic and brain tissues, and production of reactive oxygen species by peritoneal macrophages were assessed. In addition, mice were fed a zinc-deficient diet for 5 weeks, and treated with TPA and/or zinc l -methionine or zinc dl -methionine, and similar studies were conducted. 2. 2. The zinc-deficient diet induced oxidative stress in the hepatic and brain tissues as well as in the peritoneal macrophages as evidenced by significantly enhanced lipid peroxidation, DNA fragmentation, glutathione depletion, and production of reactive oxygen species. 3. 3. Treatment of mice with zinc l -methionine, zinc dl -methionine, and VES decreased TPA-induced reactive oxygen species production as evidenced by significant decreases in chemiluminescence in peritoneal macrophages by approximately 45%, 31%, and 47%, respectively, and cytochrome c reduction by approximately 54%, 35%, and 41%, respectively, as compared with control values. Similar results were observed with liver and brain lipid peroxidation, DNA fragmentation, and glutathione depletion. 4. 4. Zinc salts and antioxidants provided significant protection against TPA-induced oxidative damage. Zinc dl -methionine provided the best protection. |
| Databáze: | OpenAIRE |
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