Examination of the human motor endplate after brachial plexus injury with two‐photon microscopy
Autor: | Sameer B. Shah, Ranjan Gupta, Jeffery D. Kocsis, Justin P. Chan, James Clune, Tahseen Mozaffar, Samuel R. Ward, Oswald Steward |
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Rok vydání: | 2019 |
Předmět: |
Adult
Male 0301 basic medicine Wallerian degeneration Pathology medicine.medical_specialty Physiology Deltoid curve Neural Conduction 030105 genetics & heredity Motor Endplate Neuromuscular junction Young Adult 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Physiology (medical) medicine Humans Brachial Plexus Neuropathies Microscopy Electromyography business.industry Optical Imaging Deltoid Muscle Nerve injury medicine.disease medicine.anatomical_structure Brachial plexus injury Immunohistochemistry Neurology (clinical) Gunshot wound medicine.symptom business 030217 neurology & neurosurgery |
Zdroj: | Muscle & Nerve. 61:390-395 |
ISSN: | 1097-4598 0148-639X |
Popis: | INTRODUCTION After traumatic nerve injury, neuromuscular junction remodeling plays a key role in determining functional outcomes. Immunohistochemical analyses of denervated muscle biopsies may provide valuable prognostic data regarding clinical outcomes to supplement electrodiagnostic studies. METHODS We performed biopsies on nonfunctioning deltoid muscles in two patients after gunshot wounds and visualized the neuromuscular junctions using two-photon microscopy with immunohistochemistry. RESULTS Although the nerves in both patients showed evidence of acute Wallerian degeneration, some of the motor endplates were intact but exhibited significantly decreased surface area and volume. Both patients exhibited substantial recovery of motor function over several weeks postinjury. DISCUSSION Two-photon microscopic assessment of neuromuscular junction integrity and motor endplate morphometry in muscle biopsies provided evidence of partial sparing of muscle innervation. This finding supported the clinical judgment that eventual recovery would occur. With further study, this technique may help to guide operative decisionmaking after traumatic nerve injuries. |
Databáze: | OpenAIRE |
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