Combined prime-boost vaccination against tick-borne encephalitis (TBE) using a recombinant vaccinia virus and a bacterial plasmid both expressing TBE virus non-structural NS1 protein
Autor: | J. R. Stephenson, Ludmila Zakharova, Anatoly D. Altstein, Mikhail V. Khoretonenko, G. V. Pashvykina, AV Timofeev, S. E. Aleshin, Alexander M. Shneider |
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Jazyk: | angličtina |
Rok vydání: | 2005 |
Předmět: |
Microbiology (medical)
lcsh:QR1-502 Immunization Secondary Heterologous Cytomegalovirus Vaccinia virus Viral Nonstructural Proteins Recombinant virus Microbiology Virus lcsh:Microbiology Encephalitis Viruses Tick-Borne Lethal Dose 50 03 medical and health sciences Mice 0302 clinical medicine medicine Vaccines DNA Animals Humans 030304 developmental biology 0303 health sciences Mice Inbred BALB C biology Bacteria Viral Vaccine Tick-borne encephalitis Viral Vaccines medicine.disease biology.organism_classification Virology Vaccination Flavivirus Immunization Encephalitis Tick-Borne 030215 immunology Research Article Plasmids |
Zdroj: | BMC Microbiology BMC Microbiology, Vol 5, Iss 1, p 45 (2005) |
ISSN: | 1471-2180 |
Popis: | BackgroundHeterologous prime-boost immunization protocols using different gene expression systems have proven to be successful tools in protecting against various diseases in experimental animal models. The main reason for using this approach is to exploit the ability of expression cassettes to prime or boost the immune system in different ways during vaccination procedures. The purpose of the project was to study the ability of recombinant vaccinia virus (VV) and bacterial plasmid, both carrying the NS1 gene from tick-borne encephalitis (TBE) virus under the control of different promoters, to protect mice against lethal challenge using a heterologous prime-boost vaccination protocol.ResultsThe heterologous prime-boost vaccination protocol, using a VV recombinant and bacterial plasmid, both containing the NS1 TBE virus protein gene under the control of different promoters, achieved a high level of protection in mice against lethal challenge with a highly pathogenic TBE virus strain. No signs of pronounced TBE infection were detected in the surviving animals.ConclusionHeterologous prime-boost vaccination protocols using recombinant VV and bacterial plasmids could be used for the development of flavivirus vaccines. |
Databáze: | OpenAIRE |
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