Hybrid adaptive and computational light-sheet fluorescence microscopy
Autor: | Wilding, D., Pozzi, P., Soloviev, O.A., Vdovin, Gleb, Fiolka, Reto, Verhaegen, M.H.G., Bifano, T.G., Kubby, J., Gigan, S. |
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Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
Microscope
Materials science Optical sectioning business.industry Scattering 010401 analytical chemistry light-sheet deconvolution 01 natural sciences Fluorescence 0104 chemical sciences law.invention 010309 optics Optics law Light sheet fluorescence microscopy 0103 physical sciences Microscopy Fluorescence microscope microscopy business Adaptive optics |
Zdroj: | Proceedings of SPIE: Adaptive Optics and Wavefront Control for Biological Systems IV, 10502 |
Popis: | The light-sheet fluorescence microscopy is an excellent tool for the investigation of large three dimensional microscopy samples at the cellular level, however, the ability to resolve features is strongly affected by the presence of scattering and aberrations. These effects are two fold in light-sheet microscopy, as the illumination path providing the optical sectioning and the fluorescence detection path are both affected by the aberrations in different ways. To overcome these difficulties, we have developed hybrid adaptive optical and computational microscopy techniques to remove the effect of the aberrations in both the excitation and the fluorescence paths of these microscopes. |
Databáze: | OpenAIRE |
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