Vasopressin increases type IV collagen production through the induction of transforming growth factor-beta secretion in rat mesangial cells
| Autor: | Yuichi Tomura, Junko Tsukada, Takeyuki Yatsu, Atsuo Tahara, Masayuki Shibasaki |
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| Rok vydání: | 2008 |
| Předmět: |
Collagen Type IV
Male medicine.medical_specialty Morpholines Extracellular matrix Type IV collagen Piperidines Transforming Growth Factor beta Internal medicine medicine Animals Spiro Compounds Secretion Transforming growth factor-beta secretion Rats Wistar Autocrine signalling Receptor Cells Cultured Pharmacology Dose-Response Relationship Drug Mesangial cell biology Chemistry Transforming growth factor beta Benzazepines Rats Arginine Vasopressin Endocrinology Mesangial Cells biology.protein Antidiuretic Hormone Receptor Antagonists hormones hormone substitutes and hormone antagonists |
| Zdroj: | Pharmacological Research. 57:142-150 |
| ISSN: | 1043-6618 |
| DOI: | 10.1016/j.phrs.2008.01.003 |
| Popis: | Production of extracellular matrix proteins, such as type IV collagen, by mesangial cells contributes to progressive glomerulosclerosis. Transforming growth factor-beta (TGF-beta) modulates mesangial cell growth and stimulates extracellular matrix synthesis by mesangial cells. In this study, the ability of vasopressin (AVP), which causes mesangial cell proliferation and hypertrophy, to stimulate type IV collagen production and correlation with TGF-beta secretion by cultured rat mesangial cells was examined. AVP induced a time- and concentration-dependent increase in TGF-beta secretion and mitogenic effect in rat mesangial cells. This AVP-induced increase in TGF-beta secretion was potently inhibited by AVP V(1A) receptor-selective antagonist. AVP also induced a concentration-dependent increase in the production of type IV collagen and this effect was inhibited by V(1A) receptor-selective antagonist. Furthermore, TGF-beta also induced an increase in the production of type IV collagen; the AVP-enhanced production of type IV collagen was inhibited by an anti-TGF-beta antibody. These results demonstrate that AVP stimulates synthesis of type IV collagen by cultured rat mesangial cells through the induction of TGF-beta synthesis mediated by V(1A) receptors. Therefore, AVP-induced TGF-beta secretion by proliferating mesangial cells might act as an autocrine factor to regulate synthesis of extracellular matrix; this mechanism may contribute to glomerulosclerosis in renal diseases including diabetic nephropathy. |
| Databáze: | OpenAIRE |
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