HECT ubiquitin ligase Smurf1 targets the tumor suppressor ING2 for ubiquitination and degradation
| Autor: | Chunyan Tian, Shan He, Jing Nie, Fuchu He, Lingqiang Zhang, Min Wu, Yuxin Yin, Lin Liu, Guichun Xing |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
HECT domain
Proteasome Endopeptidase Complex Inhibitor of growth 2 Biochemical Phenomena Biophysics NEDD4 SMAD Protein degradation Biochemistry law.invention Ligases Ubiquitin Structural Biology law Genetics Humans Genes Tumor Suppressor Molecular Biology biology Chemistry Ubiquitination Tumor suppressor Cell Biology Molecular biology Growth Inhibitors Cell biology Ubiquitin ligase biology.protein Smad ubiquitination regulatory factor 1 Suppressor Binding domain |
| Zdroj: | FEBS letters. 584(14) |
| ISSN: | 1873-3468 |
| Popis: | Inhibitor of growth 2 (ING2) gene encodes a candidate tumor suppressor and is frequently reduced in many tumors. However, the mechanisms underlying the regulation of ING2, in particular its protein stability, are still unclear. Here we show that the homologous to E6AP carboxyl terminus (HECT)-type ubiquitin ligase Smad ubiquitination regulatory factor 1 (Smurf1) interacts with and targets ING2 for poly-ubiquitination and proteasomal degradation. Intriguingly, the ING2 binding domain in Smurf1 was mapped to the catalytic HECT domain. Furthermore, the C-terminal PHD domain of ING2 was required for Smurf1-mediated degradation. This study provided the first evidence that the stability of ING2 could be regulated by ubiquitin-mediated degradation.Structured summaryMINT-7894271: ING2 (uniprotkb:Q9H160) binds (MI:0407) to Smurf1 (uniprotkb:Q9HCE7) by pull-down (MI:0096)MINT-7894319, MINT-7894339: ING2 (uniprotkb:Q9H160) physically interacts (MI:0915) with Smurf1 (uniprotkb:Q9HCE7) by anti tag co-immunoprecipitation (MI:0007)MINT-7894301: Smurf1 (uniprotkb:Q9HCE7) physically interacts (MI:0915) with ING2 (uniprotkb:Q9H160) by anti bait co-immunoprecipitation (MI:0006)MINT-7894358: ING1b (uniprotkb:Q9UK53-2) physically interacts (MI:0915) with Smurf1 (uniprotkb:Q9HCE7) by anti tag co-immunoprecipitation (MI:0007)MINT-7894249: ING2 (uniprotkb:Q9H160) physically interacts (MI:0915) with ubiquitin (uniprotkb:P62988) by anti tag co-immunoprecipitation (MI:0007) |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text