Better than bench top. High speed antioxidant screening via the cupric reducing antioxidant capacity reagent and reaction flow chromatography
| Autor: | Arianne Soliven, R. Andrew Shalliker, Andrew Jones, Agustín Acquaviva, Thirada Suktham, Gary R. Dennis |
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| Rok vydání: | 2020 |
| Předmět: |
Antioxidant
medicine.medical_treatment 02 engineering and technology Raw material 01 natural sciences High-performance liquid chromatography post column derivatisation Analytical Chemistry purl.org/becyt/ford/1 [https] REACTION FLOW CHROMATOGRAPHY chemistry.chemical_compound purl.org/becyt/ford/1.4 [https] medicine POST COLUMN DERIVATISATION CUPRIC REDUCING ANTIOXIDANT CAPACITY 96 well plate Derivatization Ciencias Exactas Spectroscopy TOTAL ANTIOXIDANT CAPACITY cupric reducing antioxidant capacity Chromatography Chemistry SELECTIVE DETECTION 010401 analytical chemistry Química 021001 nanoscience & nanotechnology selective detection 0104 chemical sciences Antioxidant capacity Reagent total antioxidant capacity Natural Product Research 0210 nano-technology reaction flow chromatography |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| ISSN: | 0026-265X |
| Popis: | This study is based upon a recently established method for quantification of the antioxidant capacity of natural samples via a HPLC separation and a hyphenated selective detection (post-column derivatization with cupric reducing antioxidant capacity reagent) technique. This protocol demonstrated the main improvements to transform the quantitative protocol into a high-speed qualitative automated assay to screen samples for their potential total antioxidant capacity, typically performed via manual mixing of the sample and derivatisation and measured on a 96 well plate reader/bench top UV–Vis spectrophotometer. This approach with automated mixing is a more informative alternative for total antioxidant capacity as the antioxidant peaks are profiled for each sample within four minutes. This antioxidant profile may be used for routine analysis of raw materials and/or a guide for targeted approaches for structure elucidation for laboratories interested in early drug discovery, natural product research and the search of alternative antioxidant additives in consumer goods/therapeutics. This technique could also be used to monitor the stability, alteration or adulteration of manufactured goods containing antioxidants. Laboratorio de Investigación y Desarrollo de Métodos Analíticos (LIDMA) |
| Databáze: | OpenAIRE |
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