Cross-reactive microbial peptides can modulate HIV-specific CD8+ T cell responses

Autor: Walt Lichmira, Robert F. Siliciano, Daniel C. Xu, Megan E. May, Joel N. Blankson, M. Sue Leffell, Sarah B. Laskey, Nicholas J. Butler, Adam A. Capoferri, Caroline C. Garliss, Christopher W. Pohlmeyer, John A. Flynn, Alison G. Livingston, Richard D. Moore, Jennifer E. Thorne, Sarah E. Beck
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
RNA viruses
lcsh:Medicine
HIV Infections
CD8-Positive T-Lymphocytes
Pathology and Laboratory Medicine
Virus Replication
Epitope
Cell-Mediated Immunity
White Blood Cells
Spectrum Analysis Techniques
Immunodeficiency Viruses
Animal Cells
Medicine and Health Sciences
Cytotoxic T cell
lcsh:Science
Immune Response
Staining
Multidisciplinary
Chemistry
T Cells
virus diseases
Cell Staining
Acquired immune system
Flow Cytometry
3. Good health
Cell biology
Medical Microbiology
Spectrophotometry
Viral Pathogens
Viruses
Cytophotometry
Cellular Types
Pathogens
Research Article
Immune Cells
Immunology
Cytotoxic T cells
Cross Reactions
Research and Analysis Methods
Peripheral blood mononuclear cell
Microbiology
03 medical and health sciences
Immune system
Antigen
Immunity
Retroviruses
Humans
Molecular Biology Techniques
Microbial Pathogens
Molecular Biology
Blood Cells
lcsh:R
Lentivirus
Organisms
Biology and Life Sciences
HIV
Cell Biology
030104 developmental biology
Specimen Preparation and Treatment
HIV-1
lcsh:Q
CD8
Cloning
Zdroj: PLoS ONE
PLoS ONE, Vol 13, Iss 2, p e0192098 (2018)
ISSN: 1932-6203
Popis: Heterologous immunity is an important aspect of the adaptive immune response. We hypothesized that this process could modulate the HIV-1-specific CD8+ T cell response, which has been shown to play an important role in HIV-1 immunity and control. We found that stimulation of peripheral blood mononuclear cells (PBMCs) from HIV-1-positive subjects with microbial peptides that were cross-reactive with immunodominant HIV-1 epitopes resulted in dramatic expansion of HIV-1-specific CD8+ T cells. Interestingly, the TCR repertoire of HIV-1-specific CD8+ T cells generated by ex vivo stimulation of PBMCs using HIV-1 peptide was different from that of cells stimulated with cross-reactive microbial peptides in some HIV-1-positive subjects. Despite these differences, CD8+ T cells stimulated with either HIV-1 or cross-reactive peptides effectively suppressed HIV-1 replication in autologous CD4+ T cells. These data suggest that exposure to cross-reactive microbial antigens can modulate HIV-1-specific immunity.
Databáze: OpenAIRE
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