Antimicrobial Effects of Potential Probiotics of Bacillus spp. Isolated from Human Microbiota: In Vitro and In Silico Methods
| Autor: | Ángel Ruiz-Moreno, Klara Cerk, Jesús Pardo-Cacho, Margarita Aguilera, Alfonso Torres-Sánchez, Ana López-Moreno |
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| Přispěvatelé: | [Torres-Sánchez,A, Pardo-Cacho,J, López-Moreno,A, Ruiz-Moreno,Á, Cerk,K, Aguilera,M] Department of Microbiology, Faculty of Pharmacy, University of Granada, Campus of Cartuja, Granada, Spain. [Torres-Sánchez,A, Aguilera,M] Institute of Nutrition and Food Technology 'José Mataix', CIBM, University of Granada, Granada, Spain. [López-Moreno,A, Aguilera,M] Instituto de Investigación Biosanitaria ibs (IBS), Granada, Spain., This work was carried out within the frame of FEDER-Infrastructure Project: IE_2019-198 and OBEMIRISK EFSA-Partnering Grant Project GP/EFSA/ENCO/2018/03-GA04. A.T.-S. and Á.R.-M. 'Colaboración Investigación—Master'. J.P.-C. 'ICARO-Extracurriculares Prácticas'. A.L.-M. 'Incentivación de la Investigación'. Plan Propio-UGR. K.C. is collaborating with UGR under the EU-FORA Programme (2020/2021). |
| Rok vydání: | 2021 |
| Předmět: |
Técnicas in vitro
0301 basic medicine Microbiology (medical) in silico methods QH301-705.5 Technology and Food and Beverages::Food and Beverages::Food::Dietary Supplements::Probiotics [Medical Subject Headings] Organisms::Bacteria::Gram-Negative Bacteria::Gram-Negative Aerobic Bacteria::Gram-Negative Aerobic Rods and Cocci::Pseudomonadaceae::Pseudomonas [Medical Subject Headings] In silico 030106 microbiology Bacillus medicine.disease_cause Microbiology Genome Article Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings] 03 medical and health sciences Anti-Infective agents Virology antimicrobial effect medicine Biology (General) probiotics in vitro methods Escherichia coli Organisms::Bacteria::Endospore-Forming Bacteria::Gram-Positive Endospore-Forming Bacteria::Gram-Positive Endospore-Forming Rods::Bacillaceae::Bacillus [Medical Subject Headings] Information Science::Information Science::Information Storage and Retrieval::Data Mining [Medical Subject Headings] biology Organisms::Bacteria::Endospore-Forming Bacteria::Gram-Positive Endospore-Forming Bacteria::Gram-Positive Endospore-Forming Rods::Staphylococcaceae::Staphylococcus::Staphylococcus aureus [Medical Subject Headings] Pseudomonas Probióticos Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Physiological Effects of Drugs::Endocrine Disruptors [Medical Subject Headings] Human microbiome biology.organism_classification Antimicrobial Simulación por ordenador 030104 developmental biology Disciplines and Occupations::Natural Science Disciplines::Biological Science Disciplines::Biology::Computational Biology [Medical Subject Headings] Disciplines and Occupations::Natural Science Disciplines::Biological Science Disciplines::Biotechnology [Medical Subject Headings] Serratia marcescens Organisms::Bacteria::Gram-Positive Bacteria::Gram-Positive Cocci::Streptococcaceae::Streptococcus::Streptococcus pyogenes [Medical Subject Headings] |
| Zdroj: | Digibug. Repositorio Institucional de la Universidad de Granada instname Microorganisms; Volume 9; Issue 8; Pages: 1615 Microorganisms, Vol 9, Iss 1615, p 1615 (2021) Microorganisms |
| ISSN: | 2076-2607 |
| DOI: | 10.3390/microorganisms9081615 |
| Popis: | This work was carried out within the frame of FEDER-Infrastructure Project: IE_2019-198 and OBEMIRISK EFSA-Partnering Grant Project GP/EFSA/ENCO/2018/03-GA04. A.T.-S. and a.R.-M. "Colaboracion Investigacion-Master". J.P.-C. "ICARO-Extracurriculares Practicas". A.L.-M. "Incentivacion de la Investigacion". Plan Propio-UGR. K.C. is collaborating with UGR under the EU-FORA Programme (2020/2021). The variable taxa components of human gut microbiota seem to have an enormous biotechnological potential that is not yet well explored. To investigate the usefulness and applications of its biocompounds and/or bioactive substances would have a dual impact, allowing us to better understand the ecology of these microbiota consortia and to obtain resources for extended uses. Our research team has obtained a catalogue of isolated and typified strains from microbiota showing resistance to dietary contaminants and obesogens. Special attention was paid to cultivable Bacillus species as potential next-generation probiotics (NGP) together with their antimicrobial production and ecological impacts. The objective of the present work focused on bioinformatic genome data mining and phenotypic analyses for antimicrobial production. In silico methods were applied over the phylogenetically closest type strain genomes of the microbiota Bacillus spp. isolates and standardized antimicrobial production procedures were used. The main results showed partial and complete gene identification and presence of polyketide (PK) clusters on the whole genome sequences (WGS) analysed. Moreover, specific antimicrobial effects against B. cereus, B. circulans, Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Serratia marcescens, Klebsiella spp., Pseudomonas spp., and Salmonella spp. confirmed their capacity of antimicrobial production. In conclusion, Bacillus strains isolated from human gut microbiota and taxonomic group, resistant to Bisphenols as xenobiotics type endocrine disruptors, showed parallel PKS biosynthesis and a phenotypic antimicrobial effect. This could modulate the composition of human gut microbiota and therefore its functionalities, becoming a predominant group when high contaminant exposure conditions are present. European Commission IE_2019-198 OBEMIRISK EFSA GP/EFSA/ENCO/2018/03-GA04 |
| Databáze: | OpenAIRE |
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